DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, SJ | ko |
dc.contributor.author | Park, JP | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-09 | - |
dc.date.available | 2010-12-09 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-02 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.24, no.3, pp.185 - 189 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20861 | - |
dc.description.abstract | Production of poly(3-hydroxybutyrate) [P(3HB)] from whey by fed-batch culture of recombinant Escherichia coli CGSC 4401 harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes was examined in a 30 l fermenter supplying air only. With lactose below 2 g l(-1), cells grew to 12 g dry cell l(-1) with 9% (w/w) P(3HB) content. Accumulation of P(3HB) could be triggered by increasing lactose to 20 g l(-1). By employing this strategy, 51 g dry cell l(-1) was obtained with a 70% (w/w) P(3HB) content after 26 h. The productivity was 1.35 g P(3HB) l(-1) h(-1). The same fermentation strategy was used in a 300 l fermenter, and 30 g dry cell l(-1) with 67% (w/w) P(3HB) content was obtained in 20 h. | - |
dc.description.sponsorship | We thank Mary Berlyn for kindly providing the CGSC strain. This work was supported by the Korea- Australia International Cooperative Project from the Korean Ministry of Science and Technology. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.title | Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter | - |
dc.type | Article | - |
dc.identifier.wosid | 000173802700006 | - |
dc.identifier.scopusid | 2-s2.0-0036160292 | - |
dc.type.rims | ART | - |
dc.citation.volume | 24 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 185 | - |
dc.citation.endingpage | 189 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, SJ | - |
dc.contributor.nonIdAuthor | Park, JP | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | pilot-scale fermenter | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyrate) | - |
dc.subject.keywordAuthor | recombinant | - |
dc.subject.keywordAuthor | E. coli | - |
dc.subject.keywordAuthor | whey | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATE PRODUCTION | - |
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