Enhanced production of succinic acid by metabolically engineered Escherichia coli with amplified activities of malic enzyme and fumarase

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dc.contributor.authorHong, Soon Hoko
dc.contributor.authorLee, Sang Yupko
dc.date.accessioned2010-12-06T07:06:38Z-
dc.date.available2010-12-06T07:06:38Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-07-
dc.identifier.citationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.9, no.4, pp.252 - 255-
dc.identifier.issn1226-8372-
dc.identifier.urihttp://hdl.handle.net/10203/20761-
dc.description.abstractA pfl IdhA double mutant Escherichia coli strain NZN111 was used to produce succinic acid by overexpressing the E. coli malic enzyme gene (sfcA). This strain, however, produced a large amount of malic acid as well as succinic acid. After the analyses of the metabolic pathways, the fumB gene encoding the anaerobic fumarase of E. coli was co-amplified to solve the problem of malic acid accumulation. A plasmid, pTrcMLFu, was constructed, which contains an artificial operon (sfcA-fumB) under the control of the inducible trc promoter. From the batch culture of recombinant E. coli NZN111 harboring pTrcMLFu, 7 g/L of succinic acid was produced from 20 g/L of glucose, with no accumulation of malic acid. From the metabolic flux analysis the strain was found under reducing power limiting conditions by severe reorientation of metabolic fluxes.-
dc.description.sponsorshipWe thank David P. Clark for kindly providing the E.coli strain NZN111. This work was financially supported by the Korean Systems Biology Research Program (M10309020000-03B5002-00000) of the Ministry of Science and Technology (MOST). Further supports by the IBM-SUR program, LG Chem Chair Professorship and BK21 program are appreciated.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherKOREAN SOC BIOTECHNOLOGY & BIOENGINEERING-
dc.titleEnhanced production of succinic acid by metabolically engineered Escherichia coli with amplified activities of malic enzyme and fumarase-
dc.typeArticle-
dc.identifier.wosid000223710100003-
dc.identifier.scopusid2-s2.0-28344441063-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.issue4-
dc.citation.beginningpage252-
dc.citation.endingpage255-
dc.citation.publicationnameBIOTECHNOLOGY AND BIOPROCESS ENGINEERING-
dc.identifier.doi10.1007/BF02942339-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, Sang Yup-
dc.contributor.nonIdAuthorHong, Soon Ho-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorsuccinic acid-
dc.subject.keywordAuthormalic enzyme-
dc.subject.keywordAuthorfumarase-
dc.subject.keywordAuthormetabolic engineering-
dc.subject.keywordPlusFLUX ANALYSIS-
dc.subject.keywordPlusOVEREXPRESSION-
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