DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Y | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-06T05:23:26Z | - |
dc.date.available | 2010-12-06T05:23:26Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-04 | - |
dc.identifier.citation | JOURNAL OF ENVIRONMENTAL POLYMER DEGRADATION, v.4, no.2, pp.131 - 134 | - |
dc.identifier.issn | 1064-7546 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20755 | - |
dc.description.abstract | Fed-batch cultures of recombinant Escherichia coli strains were carried out for the production of poly(3-hydroxybutyric acid) (PHB) in a chemically defined medium. The E. coli strains used were XL1-Blue, harboring pSYL105, a stable high-copy number plasmid containing the Alcaligenes eutrophus polyhydroxyalkanoate (PHA) genes, and XL1-Blue, harboring pSYL107; which is pSYL105 containing the E. coli ftsZ gene to suppress filamentation. With XL1-Blue(pSYL105) the final cell mass and PHB concentration obtained in 62 h were 102 and 22.5 g/L, respectively. Fed-batch culture of XL1-Blue(pSYL107) under identical conditions resulted in a final cell mass and PHB concentration of 127.5 and 48.2 g/L, respectively. The PHB contents obtained with XL1-Blue(pSYL105) and XL1-Blue(pSYL107) were 22.1 and 37.8%, respectively. Therefore, PHB was more efficiently produced in a defined medium by employing filamentation-suppressed recombinant E. coli. | - |
dc.description.sponsorship | We would like to thank Prof. H. N. Chang and Prof. Y. K. Chang for various support. Part of this work was presented at Pacifichem'95, Honolulu, Hawaii. This work was supported by the G7 project of the Ministry of Science and Technology. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | PLENUM PUBL CORP | - |
dc.title | Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant Escherichia coli in a defined medium | - |
dc.type | Article | - |
dc.identifier.wosid | A1996UT34800007 | - |
dc.identifier.scopusid | 2-s2.0-0030123691 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 131 | - |
dc.citation.endingpage | 134 | - |
dc.citation.publicationname | JOURNAL OF ENVIRONMENTAL POLYMER DEGRADATION | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Lee, Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyrate) (PHB) | - |
dc.subject.keywordAuthor | filamentation | - |
dc.subject.keywordAuthor | fed-batch culture | - |
dc.subject.keywordPlus | ALCALIGENES-EUTROPHUS H16 | - |
dc.subject.keywordPlus | BETA-HYDROXYBUTYRIC ACID | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | PHB | - |
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