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College of Engineering(공과대학)Dept. of Chemical and Biomolecular Engineering(생명화학공학과)CBE-Journal Papers(저널논문)

Display of polyhistidine peptides on the Escherichia coli cell surface by using outer membrane protein C as an anchoring motif

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dc.contributor.authorXu, ZHko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2010-12-06T04:51:50Z-
dc.date.available2010-12-06T04:51:50Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1999-11-
dc.identifier.citationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.65, no.11, pp.5142 - 5147-
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10203/20747-
dc.description.abstractA novel cell surface display system was developed by employing Escherichia coli outer membrane protein C (OmpC) as an anchoring motif. Polyhistidine peptides consisting of up to 162 amino acids could be successfully displayed on the seventh exposed loop of OmpC. Recombinant cells displaying polyhistidine could adsorb up to 32.0 mu mol of Cd2+ per g (dry weight) of cells.-
dc.description.sponsorshipWe thank M.-Y. Lee and K. Beak for help with the atomic adsorption analysis and Z.-W. Lee and K.-S. Ha of the Korea Basic Science Institute for help with confocal microscopy. This work was supported by the First Young Scientist’s Award to S.Y.L. from the President of Korea and by the Korea Academy of Science and Technology. Z.X. was a visiting student from Huazhong Agricultural University, Wuhan, People’s Republic of China, and was supported in part by the BioProcess Engineering Research Center.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherAMER SOC MICROBIOLOGY-
dc.titleDisplay of polyhistidine peptides on the Escherichia coli cell surface by using outer membrane protein C as an anchoring motif-
dc.typeArticle-
dc.identifier.wosid000083478900060-
dc.identifier.scopusid2-s2.0-0344560614-
dc.type.rimsART-
dc.citation.volume65-
dc.citation.issue11-
dc.citation.beginningpage5142-
dc.citation.endingpage5147-
dc.citation.publicationnameAPPLIED AND ENVIRONMENTAL MICROBIOLOGY-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorXu, ZH-
dc.type.journalArticleArticle-
dc.subject.keywordPlusSTAPHYLOCOCCUS-CARNOSUS-
dc.subject.keywordPlusHETEROLOGOUS SEQUENCES-
dc.subject.keywordPlusEXTERNAL SURFACE-
dc.subject.keywordPlusBETA-LACTAMASE-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusLIBRARIES-
dc.subject.keywordPlusVECTOR-
dc.subject.keywordPlusYEAST-
dc.subject.keywordPlusMETALLOADSORPTION-
dc.subject.keywordPlusPOLYPEPTIDES-
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