DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mee-Jung Han | ko |
dc.contributor.author | Jeong, Kijun | ko |
dc.contributor.author | Jong-Shin Yoo | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-06T01:38:29Z | - |
dc.date.available | 2010-12-06T01:38:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-10 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.69, pp.5772 - 5781 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20727 | - |
dc.description.abstract | Variations in proteome profiles of Escherichia coli in response to the overproduction of human leptin, a serine-rich (11.6% of total amino acids) protein, were examined by two-dimensional gel electrophoresis. The levels of heat shock proteins increased, while those of protein elongation factors, 30S ribosomal protein, and some enzymes involved in amino acid biosynthesis decreased, after leptin overproduction. Most notably, the levels of enzymes involved in the biosynthesis of serine family amino acids significantly decreased. Based on this information, we designed a strategy to enhance the leptin productivity by manipulating the cysK gene, encoding cysteine synthase A. By coexpression of the cysK gene, we were able to increase the cell growth rate by approximately twofold. Also, the specific leptin productivity could be increased by fourfold. In addition, we found that cysK coexpression can improve the production of another serine-rich protein, interleukin-12 beta chain, suggesting that this strategy may be useful for the production of other serine-rich proteins as well. The approach taken in this study should be useful in designing a strategy for improving recombinant protein production. | - |
dc.description.sponsorship | This work was supported by the National Research Laboratory Program (grant 2000-N-NL-01-C-237) of the Ministry of Science and Technology; the Basic Industrial Research Project of the Ministry of Commerce, Industry and Energy; and the Brain Korea 21 project. Hardware for the analysis of proteomes was supported by the IBMSUR program. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling | - |
dc.type | Article | - |
dc.identifier.wosid | 000185881300005 | - |
dc.identifier.scopusid | 2-s2.0-0142072824 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.beginningpage | 5772 | - |
dc.citation.endingpage | 5781 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Mee-Jung Han | - |
dc.contributor.nonIdAuthor | Jong-Shin Yoo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL-DENSITY CULTURE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN | - |
dc.subject.keywordPlus | GROWTH-INHIBITION | - |
dc.subject.keywordPlus | PLASMID PRESENCE | - |
dc.subject.keywordPlus | HEAT-SHOCK | - |
dc.subject.keywordPlus | OVERPRODUCTION | - |
dc.subject.keywordPlus | REGULATOR | - |
dc.subject.keywordPlus | GENOMICS | - |
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