DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Seong Yoon | ko |
dc.contributor.author | Chung, Won-Young | ko |
dc.contributor.author | Lee, Hyeon Kyu | ko |
dc.contributor.author | Park, Myeong Soon | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2010-12-03T04:51:51Z | - |
dc.date.available | 2010-12-03T04:51:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.376, no.4, pp.633 - 636 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/20687 | - |
dc.description.abstract | The fluorogenic 1,3-Huisgen dipolar cycloaddition reaction was used as part of a novel immobilization strategy of PNA capture probes on a microarray. By using this click chemistry, azidocoumarin-anchored PNA probes were immobilized on phenyl acetylene-modified glass slides with the simultaneous generation of the fluorescent triazolycoumarin moiety. Since the emitting moieties are generated in the immobilization reaction itself, fluorescent signals can be used to directly monitor the integrity of immobilization in a nondestructive manner. By using this strategy, PNA microarrays were prepared and successfully employed to perform microarray-based diagnosis of selected Mutations in the breast cancer susceptibility gene BRCA1. (C) 2008 Elsevier Inc. All rights reserved. | - |
dc.description.sponsorship | This work was supported by the Promotional Project of Regional Science (Ministry of Science and Technology, Daejeon Metropolitan City) and the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2006-331-D00114). | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | DNA | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | MICROARRAY | - |
dc.subject | MUTATIONS | - |
dc.subject | ARRAYS | - |
dc.title | Direct and nondestructive verification of PNA immobilization using click chemistry | - |
dc.type | Article | - |
dc.identifier.wosid | 000260522300001 | - |
dc.identifier.scopusid | 2-s2.0-53449090012 | - |
dc.type.rims | ART | - |
dc.citation.volume | 376 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 633 | - |
dc.citation.endingpage | 636 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.08.147 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Lim, Seong Yoon | - |
dc.contributor.nonIdAuthor | Chung, Won-Young | - |
dc.contributor.nonIdAuthor | Lee, Hyeon Kyu | - |
dc.contributor.nonIdAuthor | Park, Myeong Soon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Click chemistry | - |
dc.subject.keywordAuthor | Immobilization | - |
dc.subject.keywordAuthor | Peptide nucleic acid | - |
dc.subject.keywordAuthor | DNA chip | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | MICROARRAY | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | ARRAYS | - |
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