High-level secretory production of human granulocyte-colony stimulating factor by fed-batch culture of recombinant Escherichia coli

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dc.contributor.authorYim, SCko
dc.contributor.authorJeong, Kijunko
dc.contributor.authorChang, Ho Namko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2010-12-02T07:58:11Z-
dc.date.available2010-12-02T07:58:11Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2001-11-
dc.identifier.citationBIOPROCESS AND BIOSYSTEMS ENGINEERING, v.24, no.4, pp.249 - 254-
dc.identifier.issn1615-7591-
dc.identifier.urihttp://hdl.handle.net/10203/20657-
dc.description.abstractSecretory production of human granulocyte colony-stimulating factor fusion protein (hG-CSF) by fed-batch culture of Escherichia coli was investigated in both 2.5-L and 30-L fermentors, To develop a fed-batch culture condition that allows efficient production of hG-CSF, different feeding strategies including pH-stat, exponential and constant feeding were examined. Among these, the constant feeding strategy (0.228 g glucosexmin(-1)) and the exponential feeding that supports a low specific growth rate (mu=0.116 h(-1)) resulted in the best hG-CSF production. Under these conditions, 4.4 gxL(-1) of hG-CSF was produced. The effect of induction time on the protein production was also investigated. For the fed-batch cultures carried out with the pH-stat and exponential feeding strategies, induction at higher cell density (late-exponential phase) resulted in more hG-CSF production compared with induction at lower cell density (early to mid-exponential phase). The constant feeding strategy that supported best hG-CSF production was applied to the scale-up production of hG-CSF in 30 L of fermentor. The maximum dry cell weight and hG-CSF concentration of 51.7 and 4.2 gxL(-1), respectively, was obtained.-
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherSPRINGER-VERLAG-
dc.titleHigh-level secretory production of human granulocyte-colony stimulating factor by fed-batch culture of recombinant Escherichia coli-
dc.typeArticle-
dc.identifier.wosid000173133500006-
dc.identifier.scopusid2-s2.0-0035660851-
dc.type.rimsART-
dc.citation.volume24-
dc.citation.issue4-
dc.citation.beginningpage249-
dc.citation.endingpage254-
dc.citation.publicationnameBIOPROCESS AND BIOSYSTEMS ENGINEERING-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorJeong, Kijun-
dc.contributor.localauthorChang, Ho Nam-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorYim, SC-
dc.type.journalArticleArticle-
dc.subject.keywordPlusCELL-DENSITY CULTURE-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusFERMENTATION-
dc.subject.keywordPlusCULTIVATION-
dc.subject.keywordPlusMETABOLISM-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusPROTEIN-
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