Engineering Corynebacterium glutamicum to overproduce 1,5-diaminopentane

Abstract

Biological production of 1,5-diaminopentane from renewable feedstock provides apromising and sustainable alternative to traditional petroleum-based chemical synthesis.In the present study, we report the development of a metabolically engineeredCorynebacterium glutamicum strain that can produce 1,5-diaminopentane from glucose.By overexpressing L-lysine decarboxylase in an industrial L-lysine overproducer C.glutamicum (U2 strain), 1,5-diaminopentane was not produced whereas L-lysine wasdetected. The cadA gene was thus codon-optimized, which led to a production titer of31.94 g/L of 1,5-diaminopentane without exogenous feeding of L-lysine by fed-batchcultivation. [This work was supported by the Technology Development Program toSolve Climate Changes on Systems Metabolic Engineering for Biorefineries from theMinistry of Science, ICT and Future Planning (MSIP) through the National ResearchFoundation (NRF) of Korea.]