DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, SJ | ko |
dc.contributor.author | Lee, Sang-Yup | ko |
dc.date.accessioned | 2010-11-30T01:45:31Z | - |
dc.date.available | 2010-11-30T01:45:31Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-04 | - |
dc.identifier.citation | MACROMOLECULAR SYMPOSIA, v.224, pp.1 - 9 | - |
dc.identifier.issn | 1022-1360 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20509 | - |
dc.description.abstract | Metabolic engineering strategies have been developed based on ever increasing molecular and genetic information of polyhydroxyalkanoate (PHA) biosynthesis in natural PHA producing bacteria, and used for developing recombinant Escherichia coli strains with enhanced PHA biosynthesis activity. Recently, systems level metabolic engineering approaches based on genomics, proteomics and fluxomics have been taken in designing an optimal bioprocess for the production of various PHAs in recombinant E. coli. For examples, in silico metabolic flux analysis (MFA) and proteome analysis revealed that several central metabolic enzymes including Eda, Fba and TpiA were amplified during poly(3-hydroxybutyrate)[P(3HB)] biosynthesis to support more acetyl-CoA and NADPH. Also, a genome informatics approach was successfully taken to identify various FadB homologous enzymes including YfcX, YdbU, PaaF and PaaG, and a new enoyl-CoA hydratase MaoC, which are involved in PHA biosynthesis from fatty acid in fadB mutant E. coli strains. These new systems level findings were employed to design metabolically engineered E. coli strains for the enhanced production of PHAs and production of novel PHAs. Therefore, systems biological approach is a robust way to improve the metabolic activities of recombinant E. coli locally as well as globally for the enhanced production of PHAs. | - |
dc.description.sponsorship | This work was supported by the Korea Systems Biology Research Grant(M10309020000-03B5002-00000). Further supports by the LG Chem Chair Professorship, IBM SUR program and by the BK21 project from the Ministry of Education are greatly appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Systems biological approach for the production of various polyhydroxyalkanoates by metabolically engineered Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000229727700002 | - |
dc.identifier.scopusid | 2-s2.0-20944449850 | - |
dc.type.rims | ART | - |
dc.citation.volume | 224 | - |
dc.citation.beginningpage | 1 | - |
dc.citation.endingpage | 9 | - |
dc.citation.publicationname | MACROMOLECULAR SYMPOSIA | - |
dc.identifier.doi | 10.1002/masy.200550601 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.nonIdAuthor | Park, SJ | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordAuthor | polyhydroxyalkanoates | - |
dc.subject.keywordAuthor | recombinant Escherichia coli | - |
dc.subject.keywordAuthor | systems biology | - |
dc.subject.keywordPlus | CHAIN-LENGTH POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | ACID BETA-OXIDATION | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | POLY(3-HYDROXYBUTYRATE) PRODUCTION | - |
dc.subject.keywordPlus | MOLECULAR CHARACTERIZATION | - |
dc.subject.keywordPlus | PROTEOME ANALYSIS | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | BACTERIAL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | REDUCTASE | - |
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