DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Myung Hyun | ko |
dc.contributor.author | Shin, Soochul | ko |
dc.contributor.author | Jung, Seung-Ryoung | ko |
dc.contributor.author | Kim, Eunji | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Hohng, Sungchul | ko |
dc.date.accessioned | 2016-04-15T03:10:18Z | - |
dc.date.available | 2016-04-15T03:10:18Z | - |
dc.date.created | 2015-06-09 | - |
dc.date.created | 2015-06-09 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | MOLECULAR CELL, v.59, no.1, pp.117 - 124 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/204016 | - |
dc.description.abstract | Argonaute is a key enzyme of various RNA silencing pathways. We use single-molecule fluorescence measurements to characterize the reaction mechanisms of the core-RISC (RNA-induced silencing complex) composed of human Argonaute 2 and a small RNA. We found that target binding of core-RISC starts at the seed region, resulting in four distinct reaction pathways: target cleavage, transient binding, stable binding, and Argonaute unloading. The target cleavage requires extensive sequence complementarity and dramatically accelerates core-RISC recycling. The stable binding of core-RISC is efficiently established with the seed match only, providing a potential explanation for the seed-match rule of miRNA (microRNA) target selection. Target cleavage on perfect-match targets sensitively depends on RNA sequences, providing an insight into designing more efficient siRNAs (small interfering RNAs). | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.subject | TRANSLATIONAL REPRESSION | - |
dc.subject | MICRORNA TARGETS | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | MOUSE OOCYTES | - |
dc.subject | GUIDE STRAND | - |
dc.subject | C-ELEGANS | - |
dc.subject | COMPLEX | - |
dc.subject | SIRNAS | - |
dc.subject | INTERFERENCE | - |
dc.subject | CLEAVAGE | - |
dc.title | Human Argonaute 2 Has Diverse Reaction Pathways on Target RNAs | - |
dc.type | Article | - |
dc.identifier.wosid | 000360987300011 | - |
dc.identifier.scopusid | 2-s2.0-84937134068 | - |
dc.type.rims | ART | - |
dc.citation.volume | 59 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 117 | - |
dc.citation.endingpage | 124 | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2015.04.027 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | Jo, Myung Hyun | - |
dc.contributor.nonIdAuthor | Shin, Soochul | - |
dc.contributor.nonIdAuthor | Jung, Seung-Ryoung | - |
dc.contributor.nonIdAuthor | Kim, Eunji | - |
dc.contributor.nonIdAuthor | Hohng, Sungchul | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TRANSLATIONAL REPRESSION | - |
dc.subject.keywordPlus | MICRORNA TARGETS | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | MOUSE OOCYTES | - |
dc.subject.keywordPlus | GUIDE STRAND | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | SIRNAS | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | CLEAVAGE | - |
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