DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Tong Un | ko |
dc.contributor.author | Kim, Won Jun | ko |
dc.contributor.author | Choi, Sol | ko |
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Lee, SY | ko |
dc.date.accessioned | 2016-04-15T03:07:28Z | - |
dc.date.available | 2016-04-15T03:07:28Z | - |
dc.date.created | 2015-09-01 | - |
dc.date.created | 2015-09-01 | - |
dc.date.created | 2015-09-01 | - |
dc.date.issued | 2015-08 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.5 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/203979 | - |
dc.description.abstract | Bio-based production of chemicals from renewable resources is becoming increasingly important for sustainable chemical industry. In this study, Escherichia coli was metabolically engineered to produce 1,3-diaminopropane (1,3-DAP), a monomer for engineering plastics. Comparing heterologous C-4 and C-5 pathways for 1,3-DAP production by genome-scale in silico flux analysis revealed that the C-4 pathway employing Acinetobacter baumannii dat and ddc genes, encoding 2-ketoglutarate 4-aminotransferase and L-2,4-diaminobutanoate decarboxylase, respectively, was the more efficient pathway. In a strain that has feedback resistant aspartokinases, the ppc and aspC genes were overexpressed to increase flux towards 1,3-DAP synthesis. Also, studies on 128 synthetic small RNAs applied in gene knock-down revealed that knocking out pfkA increases 1,3-DAP production. Overexpression of ppc and aspC genes in the pfkA deleted strain resulted in production titers of 1.39 and 1.35 g l(-1) of 1,3-DAP, respectively. Fed-batch fermentation of the final engineered E. coli strain allowed production of 13 g l(-1) of 1,3-DAP in a glucose minimal medium. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine | - |
dc.type | Article | - |
dc.identifier.wosid | 000359287500001 | - |
dc.identifier.scopusid | 2-s2.0-84939207951 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/srep13040 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SY | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PLATFORM CHEMICALS | - |
dc.subject.keywordPlus | ACINETOBACTER-BAUMANNII | - |
dc.subject.keywordPlus | BIOGENIC-AMINES | - |
dc.subject.keywordPlus | NYLON | - |
dc.subject.keywordPlus | 5-AMINOVALERATE | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DESIGN | - |
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