DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Nuri | ko |
dc.contributor.author | Kim, Kangsan | ko |
dc.contributor.author | Kim, Soo Jin | ko |
dc.contributor.author | Park, Intae | ko |
dc.contributor.author | Lee, Jung-Eun | ko |
dc.contributor.author | Seo, Young Suk | ko |
dc.contributor.author | An, Hyun Joo | ko |
dc.contributor.author | Kim, Ho Min | ko |
dc.contributor.author | Koh, Gou Young | ko |
dc.date.accessioned | 2016-04-14T02:53:38Z | - |
dc.date.available | 2016-04-14T02:53:38Z | - |
dc.date.created | 2015-11-09 | - |
dc.date.created | 2015-11-09 | - |
dc.date.created | 2015-11-09 | - |
dc.date.created | 2015-11-09 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.5 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/203694 | - |
dc.description.abstract | Angiopoietin-1 (Ang1), a potential growth factor for therapeutic angiogenesis and vascular stabilization, is known to specifically cluster and activate Tie2 in high oligomeric forms, which is a unique and essential process in this ligand-receptor interaction. However, highly oligomeric native Ang1 and Ang1 variants are difficult to produce, purify, and store in a stable and active form. To overcome these limitations, we developed a simple and active dimeric CMP-Ang1 by replacing the N-terminal of native Ang1 with the coiled-coil domain of cartilage matrix protein (CMP) bearing mutations in its cysteine residues. This dimeric CMP-Ang1 effectively increased the migration, survival, and tube formation of endothelial cells via Tie2 activation. Furthermore, dimeric CMP-Ang1 induced angiogenesis and suppressed vascular leakage in vivo. Despite its dimeric structure, the potencies of such Tie2-activation-induced effects were comparable to those of a previously engineered protein, COMP-Ang1. We also revealed that these effects of dimeric CMP-Ang1 were affected by specified N-glycosylation in its fibrinogen-like domain. Taken together, our results indicate that dimeric CMP-Ang1 is capable of activating Tie2 and stimulating angiogenesis in N-glycan dependent manner. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | A Designed Angiopoietin-1 Variant, Dimeric CMP-Ang1 Activates Tie2 and Stimulates Angiogenesis and Vascular Stabilization in N-glycan Dependent Manner | - |
dc.type | Article | - |
dc.identifier.wosid | 000362980100001 | - |
dc.identifier.scopusid | 2-s2.0-84945183684 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/srep15291 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Ho Min | - |
dc.contributor.localauthor | Koh, Gou Young | - |
dc.contributor.nonIdAuthor | Seo, Young Suk | - |
dc.contributor.nonIdAuthor | An, Hyun Joo | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | COILED-COIL | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | COMP-ANG1 | - |
dc.subject.keywordPlus | LEAKAGE | - |
dc.subject.keywordPlus | LIGAND | - |
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