DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bharmoria, Pankaj | ko |
dc.contributor.author | Trivedi, Tushar J. | ko |
dc.contributor.author | Pabbathi, Ashok | ko |
dc.contributor.author | Samanta, Anunay | ko |
dc.contributor.author | Kumar, Arvind | ko |
dc.date.accessioned | 2015-11-20T07:41:01Z | - |
dc.date.available | 2015-11-20T07:41:01Z | - |
dc.date.created | 2015-05-06 | - |
dc.date.created | 2015-05-06 | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | PHYSICAL CHEMISTRY CHEMICAL PHYSICS, v.17, no.15, pp.10189 - 10199 | - |
dc.identifier.issn | 1463-9076 | - |
dc.identifier.uri | http://hdl.handle.net/10203/200818 | - |
dc.description.abstract | Choline dioctylsulfosuccinate [Cho][AOT] (a surface active ionic liquid) has been found to induce all-alpha to alpha + beta conformational transition in the secondary structure of enzyme cytochrome c (Cyt c) with an enhanced peroxidase activity in its aqueous vesicular phase at pH 7.0. [Cho][AOT] interacted with Cyt c distinctly at three critical concentrations (aggregation C-1, saturation C-2 and vesicular C-3) as detected from isothermal titration calorimetric analysis. Oxidation of heme iron was observed from the disappearance of the Q band in the UV-vis spectra of Cyt c upon [Cho][AOT] binding above C-3. Circular dichroism analysis (CD) has shown the loss in both the secondary (190-240 nm) and tertiary (250-300 nm) structure of Cyt c in the monomeric regime until C-1, followed by their stabilization until the pre-vesicular regime (C-1 -> C-3). Loss in both the secondary and tertiary structure has been observed in the post-vesicular regime with the change in Cyt c conformation from all-alpha to alpha + beta which is similar to the conformational changes of Cyt c upon binding with mitochondrial membrane (Biochemistry 1998, 37, 6402-6409), thus citing the potential utility of [Cho][AOT] membranes as an artificial analog for in vitro bio-mimicking. Fluorescence correlation spectroscopy (FCS) measurements confirm the unfolding of Cyt c in the vesicular phase. Dynamic light scattering experiments have shown the contraction of [Cho][AOT] vesicles upon Cyt c binding driven by electrostatic interactions observed by charge neutralization from zeta potential measurements. [Cho][AOT] has been found to enhance the peroxidase activity of Cyt c with maximum activity at C-3, observed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt as the substrate in the presence of hydrogen peroxide. This result shows the relevance of tuning ionic liquids to surfactants for bio-mimicking of specific membrane protein-lipid interactions. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | BOVINE SERUM-ALBUMIN | - |
dc.subject | CIRCULAR-DICHROISM | - |
dc.subject | SURFACTANT INTERACTIONS | - |
dc.subject | ANIONIC SURFACTANTS | - |
dc.subject | MEMBRANE | - |
dc.subject | PROTEIN | - |
dc.subject | VESICLES | - |
dc.subject | BEHAVIOR | - |
dc.subject | BINDING | - |
dc.subject | BIOMEMBRANES | - |
dc.title | Ionic liquid-induced all-alpha to alpha plus beta conformational transition in cytochrome c with improved peroxidase activity in aqueous medium | - |
dc.type | Article | - |
dc.identifier.wosid | 000352270700066 | - |
dc.identifier.scopusid | 2-s2.0-84926500228 | - |
dc.type.rims | ART | - |
dc.citation.volume | 17 | - |
dc.citation.issue | 15 | - |
dc.citation.beginningpage | 10189 | - |
dc.citation.endingpage | 10199 | - |
dc.citation.publicationname | PHYSICAL CHEMISTRY CHEMICAL PHYSICS | - |
dc.identifier.doi | 10.1039/c4cp06044j | - |
dc.contributor.localauthor | Trivedi, Tushar J. | - |
dc.contributor.nonIdAuthor | Bharmoria, Pankaj | - |
dc.contributor.nonIdAuthor | Pabbathi, Ashok | - |
dc.contributor.nonIdAuthor | Samanta, Anunay | - |
dc.contributor.nonIdAuthor | Kumar, Arvind | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BOVINE SERUM-ALBUMIN | - |
dc.subject.keywordPlus | CIRCULAR-DICHROISM | - |
dc.subject.keywordPlus | SURFACTANT INTERACTIONS | - |
dc.subject.keywordPlus | ANIONIC SURFACTANTS | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | VESICLES | - |
dc.subject.keywordPlus | BEHAVIOR | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | BIOMEMBRANES | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.