Sensitive electrochemical detection of vaccinia virus in a solution containing a high concentration of L-ascorbic acid

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dc.contributor.authorPark, Seonhwako
dc.contributor.authorKim, Jihyeko
dc.contributor.authorOck, Hwiseokko
dc.contributor.authorDutta, Gorachandko
dc.contributor.authorSeo, Jeongwookko
dc.contributor.authorShin, Eui-Cheolko
dc.contributor.authorYang, Haesikko
dc.date.accessioned2015-11-20T07:38:31Z-
dc.date.available2015-11-20T07:38:31Z-
dc.date.created2015-08-25-
dc.date.created2015-08-25-
dc.date.issued2015-
dc.identifier.citationANALYST, v.140, no.16, pp.5481 - 5487-
dc.identifier.issn0003-2654-
dc.identifier.urihttp://hdl.handle.net/10203/200794-
dc.description.abstractWashing processes cannot fully remove interfering species that remain on biosensing surfaces when a sample solution contains a high concentration of interfering species. This study reports an immunosensing scheme employing electroreduction-based electrochemical-chemical (EC) redox cycling that allows sensitive detection of vaccinia virus (VV) in a solution containing a high concentration of L-ascorbic acid (AA). To obtain high signal amplification, an enzymatic reaction by beta-D-galactosidase (Gal) is combined with electroreduction-based EC redox cycling by an oxidant. Among the four possible oxidants (KIO3, NaClO, Ag2O, and H2O2), KIO3 shows the highest signal-to-background ratio and is chosen. During an incubation period of 10 min, Gal converts beta-D-galactopyranoside into p-aminophenol (AP), which is oxidized to p-quinone imine (QI) by KIO3. When -0.05 V vs. Ag/AgCl is applied to an immunosensing electrode, QI is reduced to AP, and the regenerated AP is then reoxidized by KIO3. The electroreduction-based EC redox cycling is induced. An indium-tin oxide electrode modified with reduced graphene oxide and an applied potential of -0.05 V are used to achieve low and reproducible background currents, slow O-2 reduction, and fast electroreduction of QI. KIO3 favorably converts AA into noninterfering species during the incubation period. The detection limit for VV in commercial 50% mandarin juice (AA concentration = 0.7 mM) is 4 x 10(3) plaque-forming unit (PFU) per mL. The new EC redox cycling scheme is promising for sensitive detection of proteins, viruses, and bacteria in solutions containing high concentrations of AA.-
dc.languageEnglish-
dc.publisherROYAL SOC CHEMISTRY-
dc.subjectTIN OXIDE ELECTRODES-
dc.subjectCARBON NANOTUBE-
dc.subjectINFLUENZA-VIRUS-
dc.subjectURIC-ACID-
dc.subjectBIOSENSOR-
dc.subjectDNA-
dc.subjectINTERFERENCE-
dc.subjectIMMUNOSENSOR-
dc.subjectAMPLIFICATION-
dc.subjectLABEL-
dc.titleSensitive electrochemical detection of vaccinia virus in a solution containing a high concentration of L-ascorbic acid-
dc.typeArticle-
dc.identifier.wosid000358521400010-
dc.identifier.scopusid2-s2.0-84938410561-
dc.type.rimsART-
dc.citation.volume140-
dc.citation.issue16-
dc.citation.beginningpage5481-
dc.citation.endingpage5487-
dc.citation.publicationnameANALYST-
dc.identifier.doi10.1039/c5an01086a-
dc.contributor.localauthorShin, Eui-Cheol-
dc.contributor.nonIdAuthorPark, Seonhwa-
dc.contributor.nonIdAuthorOck, Hwiseok-
dc.contributor.nonIdAuthorDutta, Gorachand-
dc.contributor.nonIdAuthorSeo, Jeongwook-
dc.contributor.nonIdAuthorYang, Haesik-
dc.type.journalArticleArticle-
dc.subject.keywordPlusTIN OXIDE ELECTRODES-
dc.subject.keywordPlusCARBON NANOTUBE-
dc.subject.keywordPlusINFLUENZA-VIRUS-
dc.subject.keywordPlusBIOSENSORS-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusIMMUNOSENSOR-
dc.subject.keywordPlusAMPLIFICATION-
dc.subject.keywordPlusLABEL-
dc.subject.keywordPlusFILM-
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