DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sunghyun | ko |
dc.contributor.author | Kim, Daejin | ko |
dc.contributor.author | Lee, Yonghyun | ko |
dc.contributor.author | Jeon, Hyungsu | ko |
dc.contributor.author | Lee, Byung-Heon | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.date.accessioned | 2015-11-20T07:38:18Z | - |
dc.date.available | 2015-11-20T07:38:18Z | - |
dc.date.created | 2015-01-27 | - |
dc.date.created | 2015-01-27 | - |
dc.date.created | 2015-01-27 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.citation | CHEMBIOCHEM, v.16, no.1, pp.43 - 46 | - |
dc.identifier.issn | 1439-4227 | - |
dc.identifier.uri | http://hdl.handle.net/10203/200792 | - |
dc.description.abstract | Affinity maturation of protein-targeting peptides is generally accomplished by homo-or heterodimerization of known peptides. However, applying a heterodimerization approach is difficult because it is not clear a priori what length or type of linker is required for cooperative binding to a target. Thus, an efficient and simple affinity maturation method for converting low-affinity peptides into high-affinity peptides would clearly be advantageous for advancing peptide-based therapeutics. Here, we describe the development of a novel affinity maturation method based on a robust beta-hairpin scaffold and combinatorial phage-display technology. With this strategy, we were able to increase the affinity of existing peptides by more than four orders of magnitude. Taken together, our data demonstrate that this scaffold-assisted approach is highly efficient and effective in generating high-affinity peptides from their low-affinity counterparts. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | RECEPTOR | - |
dc.subject | BINDING | - |
dc.subject | LIBRARIES | - |
dc.subject | DISPLAY | - |
dc.subject | DESIGN | - |
dc.subject | TARGET | - |
dc.subject | GROWTH | - |
dc.title | Conversion of Low-Affinity Peptides to High-Affinity Peptide Binders by Using a beta-Hairpin Scaffold-Assisted Approach | - |
dc.type | Article | - |
dc.identifier.wosid | 000346781600004 | - |
dc.identifier.scopusid | 2-s2.0-84919740322 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 43 | - |
dc.citation.endingpage | 46 | - |
dc.citation.publicationname | CHEMBIOCHEM | - |
dc.identifier.doi | 10.1002/cbic.201402450 | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.nonIdAuthor | Kim, Sunghyun | - |
dc.contributor.nonIdAuthor | Kim, Daejin | - |
dc.contributor.nonIdAuthor | Lee, Yonghyun | - |
dc.contributor.nonIdAuthor | Lee, Byung-Heon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | affinity maturation | - |
dc.subject.keywordAuthor | aptides | - |
dc.subject.keywordAuthor | beta-hairpin scaffold | - |
dc.subject.keywordAuthor | peptides | - |
dc.subject.keywordAuthor | phage display | - |
dc.subject.keywordAuthor | surface plasmon resonance | - |
dc.subject.keywordAuthor | affinity maturation | - |
dc.subject.keywordAuthor | aptides | - |
dc.subject.keywordAuthor | beta-hairpin scaffold | - |
dc.subject.keywordAuthor | peptides | - |
dc.subject.keywordAuthor | phage display | - |
dc.subject.keywordAuthor | surface plasmon resonance | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | DISPLAY | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | DISPLAY | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordPlus | GROWTH | - |
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