DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Kyoung-Hwan | ko |
dc.contributor.author | Park, Hyun-Gyu | ko |
dc.contributor.author | Kim, Joon-Ho | ko |
dc.contributor.author | Seong, Ki-Hun | ko |
dc.date.accessioned | 2010-11-15T06:34:40Z | - |
dc.date.available | 2010-11-15T06:34:40Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-12 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.22, no.5, pp.613 - 620 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/19919 | - |
dc.description.abstract | This paper describes fabrication of a poly(dimethyl siloxane) (PDMS)-based chip to analyze multiple protein interactions utilizing glycidyl methacrylate (GMA) photopolymer for a site-specific immobilization of capture proteins in a closed system. First, using one direction channels of a PDMS mold having cross-channels, GMA micropads were prepared by photopolymerizing GMA solution by 365 nm light irradiation at predetermined positions. After the first mold was replaced with a second mold having higher height or directly without mold changing, capture proteins were allowed to be covalently immobilized onto the surface of the epoxide-activated GMA pads. Following immobilization, poly(ethylene glycol) diacrylate (PEG-DA) precursor was photopolymerized at specific regions to generate plugs for prevention of mixing between different sample injection channels, diminishing the need of a mold changing for sample injections. Final chip was assembled by connecting separated sample injection channels using a connector mold. The viability of this strategy was successfully demonstrated by simultaneous detection of two different antigen-antibody interactions. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | This research was supported by Samsung Advanced Institute of Technology (SAIT), Center for Ultramicrochemical Process Systems sponsored by KOSEF and Brain Korea 21 (BK 21) project. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | MICROFLUIDIC SYSTEMS | - |
dc.subject | FABRICATION | - |
dc.subject | DEVICES | - |
dc.subject | DNA | - |
dc.subject | EXPRESSION | - |
dc.subject | HYDROGELS | - |
dc.subject | POLYMER | - |
dc.subject | MICROSTRUCTURES | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | MICROARRAY | - |
dc.title | Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: Use of glycidyl methacrylate photopolymer for site-specific protein immobilization | - |
dc.type | Article | - |
dc.identifier.wosid | 000242230800006 | - |
dc.identifier.scopusid | 2-s2.0-33750969698 | - |
dc.type.rims | ART | - |
dc.citation.volume | 22 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 613 | - |
dc.citation.endingpage | 620 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2006.01.029 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Hyun-Gyu | - |
dc.contributor.nonIdAuthor | Kim, Joon-Ho | - |
dc.contributor.nonIdAuthor | Seong, Ki-Hun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | glycidyl methacrylate | - |
dc.subject.keywordAuthor | poly(dimethyl siloxane) (PDMS) | - |
dc.subject.keywordAuthor | microfluidics | - |
dc.subject.keywordAuthor | poly(ethylene glycol) diacrylate | - |
dc.subject.keywordAuthor | biomolecular immobilization | - |
dc.subject.keywordPlus | MICROFLUIDIC SYSTEMS | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | DEVICES | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | POLYMER | - |
dc.subject.keywordPlus | MICROSTRUCTURES | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | MICROARRAY | - |
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