DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jinwoo | ko |
dc.contributor.author | Kim, J | ko |
dc.contributor.author | Kim, J | ko |
dc.contributor.author | Jia, HF | ko |
dc.contributor.author | Kim, MI | ko |
dc.contributor.author | Kwak, JH | ko |
dc.contributor.author | Jin, SM | ko |
dc.contributor.author | Dohnalkova, A | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Wang, P | ko |
dc.contributor.author | Grate, JW | ko |
dc.contributor.author | Hyeon, T | ko |
dc.date.accessioned | 2010-11-15T01:32:33Z | - |
dc.date.available | 2010-11-15T01:32:33Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-07 | - |
dc.identifier.citation | SMALL, v.1, no.7, pp.744 - 753 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | http://hdl.handle.net/10203/19882 | - |
dc.description.abstract | Hierarchically ordered mesocellular mesoporous silica materials (HMMS) were synthesized using a single structure-directing agent. The mesocellular pores are synthesized without adding any pore expander; the pore walls are composed of SBA-15 type mesopores. Small-angle X-ray scattering revealed the presence of uniform' pore structures with two different sizes. Using HMMS as a nanoscopic template, hierarchically ordered mesocellular mesoporous carbon (HMMC) and polymer (HMMP) materials were synthesized. HMMS was used as a host for enzyme immobilization. To improve the retention of enzymes in HMMS, we adsorbed enzymes, and then employed crosslinking using glutaraldehyde (GA). The resulting crosslinked enzyme aggregates (CLEAs) show an impressive stability with extremely high enzyme loadings. For example, 0.5 g alpha-chymotrypsin (CT) could be loaded in 1 g of silica with no activity decrease observed with rigorous shaking over one month. In contrast, adsorbed CT without GA treatment resulted in a lower loading, which further decreased due to continuous leaching of adsorbed CT under shaking. The activity of crosslinked CT aggregates in HMMS was approximate to 10 times higher than that of the adsorbed CT, which represents a 74-fold increase in activity per unit weight of HMMS due to higher CT loading. | - |
dc.description.sponsorship | T.H. is grateful for financial support from the Korean Ministry of Science and Technology through the National Creative Research Initiative Program. J.K. would like to thank U.S. Department of Energy (DOE) LDRD funds administered by the Pacific Northwest National Laboratory, and the DOE Office of Biological and Environmental Research under the Environmental Management Science Program. The research was performed in part at the W. R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy’s Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory. We thank Prof. Chae-Ho Shin at the Chungbuk National University for the micropore structure characterization. We also thank Dr. Hyunmin Park at the Korea Research Institute of Standards and Science for the small-angle X-ray scattering studies. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | CHEMICAL-VAPOR-DEPOSITION | - |
dc.subject | CARBON MOLECULAR-SIEVES | - |
dc.subject | CAGE-LIKE PORES | - |
dc.subject | POROUS-SILICA | - |
dc.subject | METAL-OXIDES | - |
dc.subject | TRIBLOCK COPOLYMER | - |
dc.subject | NANOPOROUS CARBON | - |
dc.subject | POLYMER FOAMS | - |
dc.subject | LOW-COST | - |
dc.subject | TEMPLATES | - |
dc.title | Simple synthesis of hierarchically ordered mesocellular mesoporous silica materials hosting crosslinked enzyme aggregates | - |
dc.type | Article | - |
dc.identifier.wosid | 000229876900014 | - |
dc.identifier.scopusid | 2-s2.0-27844571788 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 744 | - |
dc.citation.endingpage | 753 | - |
dc.citation.publicationname | SMALL | - |
dc.identifier.doi | 10.1002/smll.200500035 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Jinwoo | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.nonIdAuthor | Kim, J | - |
dc.contributor.nonIdAuthor | Kim, J | - |
dc.contributor.nonIdAuthor | Jia, HF | - |
dc.contributor.nonIdAuthor | Kim, MI | - |
dc.contributor.nonIdAuthor | Kwak, JH | - |
dc.contributor.nonIdAuthor | Jin, SM | - |
dc.contributor.nonIdAuthor | Dohnalkova, A | - |
dc.contributor.nonIdAuthor | Wang, P | - |
dc.contributor.nonIdAuthor | Grate, JW | - |
dc.contributor.nonIdAuthor | Hyeon, T | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | crosslinking | - |
dc.subject.keywordAuthor | enzymes | - |
dc.subject.keywordAuthor | mesoporous materals | - |
dc.subject.keywordAuthor | silica | - |
dc.subject.keywordAuthor | template synthesis | - |
dc.subject.keywordPlus | CHEMICAL-VAPOR-DEPOSITION | - |
dc.subject.keywordPlus | CARBON MOLECULAR-SIEVES | - |
dc.subject.keywordPlus | CAGE-LIKE PORES | - |
dc.subject.keywordPlus | POROUS-SILICA | - |
dc.subject.keywordPlus | METAL-OXIDES | - |
dc.subject.keywordPlus | TRIBLOCK COPOLYMER | - |
dc.subject.keywordPlus | NANOPOROUS CARBON | - |
dc.subject.keywordPlus | POLYMER FOAMS | - |
dc.subject.keywordPlus | LOW-COST | - |
dc.subject.keywordPlus | TEMPLATES | - |
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