DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yun Sok | ko |
dc.contributor.author | Koh, Hae-Young | ko |
dc.contributor.author | Park, Sangdai | ko |
dc.contributor.author | Kim, JaeBum | ko |
dc.date.accessioned | 2015-04-29T02:33:37Z | - |
dc.date.available | 2015-04-29T02:33:37Z | - |
dc.date.created | 2015-01-09 | - |
dc.date.created | 2015-01-09 | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Korean Journal of Biological Sciences, v.8, no.1, pp.49 - 55 | - |
dc.identifier.issn | 1226-5071 | - |
dc.identifier.uri | http://hdl.handle.net/10203/198384 | - |
dc.description.abstract | In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell-type- and gene-specific DNA-binding proteins in a chromatin-selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin-dependent gene expression through binding to SRE and E-box motif with dual DNA binding specificity. Although its transcriptional and post-translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SEBP1c, we employed the GST pull-down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREP1c protein. GST pull-down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co-transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c | - |
dc.language | English | - |
dc.publisher | Korean Association of Biological Sciences | - |
dc.title | Identification of Ku70/Ku80 as ADD1/SREBP1c Interacting Proteins | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 49 | - |
dc.citation.endingpage | 55 | - |
dc.citation.publicationname | Korean Journal of Biological Sciences | - |
dc.contributor.localauthor | Lee, Yun Sok | - |
dc.contributor.nonIdAuthor | Koh, Hae-Young | - |
dc.contributor.nonIdAuthor | Park, Sangdai | - |
dc.contributor.nonIdAuthor | Kim, JaeBum | - |
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