DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, HJ | ko |
dc.contributor.author | Park, J | ko |
dc.contributor.author | Lee, HW | ko |
dc.contributor.author | Lee, Yun Sok | ko |
dc.contributor.author | Kim, JaeBum | ko |
dc.date.accessioned | 2015-04-29T02:33:12Z | - |
dc.date.available | 2015-04-29T02:33:12Z | - |
dc.date.created | 2015-01-06 | - |
dc.date.created | 2015-01-06 | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.321, no.4, pp.942 - 948 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/198382 | - |
dc.description.abstract | Here, we demonstrated that inhibition of mTOR with rapamycin has negative effects on adipocyte differentiation and insulin signaling. Rapamycin significantly reduced expression of most adipocyte marker genes including PPARgamma, adipsin, aP2, ADD1/SREBP1c, and FAS, and decreased intracellular lipid accumulation in 3T3-L1 and 3T3-F442A cells, suggesting that rapamycin would affect both lipogenesis and adipogenesis. Contrary to the previous report that suppressive effect of rapamycin on adipogenesis is limited to the clonal expansion, we revealed that its inhibitory effect persisted throughout the process of adipocyte differentiation. Thus, it is likely that constitutive activation of mTOR might be required for the execution of adipogenic programming. In differentiated 3T3-L1 adipocytes, chronic treatment of rapamycin blunted the phosphorylation of AKT and GSK, which is stimulated by insulin, and reduced insulin-dependent glucose uptake activity. Taken together, these results suggest that rapamycin not only prevents adipocyte differentiation by decrease of adipogenesis and lipogenesis but also downregulates insulin action in adipocytes, implying that mTOR would play important roles in adipogenesis and insulin action. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | MESSENGER-RNA TRANSLATION | - |
dc.subject | PROTEIN-KINASE B | - |
dc.subject | MAMMALIAN TARGET | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | 3T3-L1 ADIPOCYTES | - |
dc.subject | S6 KINASE | - |
dc.subject | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject | TRANSCRIPTIONAL REGULATION | - |
dc.subject | PHOSPHOINOSITIDE 3-KINASE | - |
dc.title | Regulation of adipocyte differentiation and insulin action with rapamycin | - |
dc.type | Article | - |
dc.identifier.wosid | 000223381100028 | - |
dc.identifier.scopusid | 2-s2.0-4043082005 | - |
dc.type.rims | ART | - |
dc.citation.volume | 321 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 942 | - |
dc.citation.endingpage | 948 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2004.07.050 | - |
dc.contributor.localauthor | Lee, Yun Sok | - |
dc.contributor.nonIdAuthor | Cho, HJ | - |
dc.contributor.nonIdAuthor | Park, J | - |
dc.contributor.nonIdAuthor | Lee, HW | - |
dc.contributor.nonIdAuthor | Kim, JaeBum | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | rapamycin | - |
dc.subject.keywordAuthor | mTOR | - |
dc.subject.keywordAuthor | adipocyte differentiation | - |
dc.subject.keywordAuthor | insulin signaling | - |
dc.subject.keywordPlus | MESSENGER-RNA TRANSLATION | - |
dc.subject.keywordPlus | PROTEIN-KINASE B | - |
dc.subject.keywordPlus | MAMMALIAN TARGET | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | 3T3-L1 ADIPOCYTES | - |
dc.subject.keywordPlus | S6 KINASE | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL REGULATION | - |
dc.subject.keywordPlus | PHOSPHOINOSITIDE 3-KINASE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.