DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, SJ | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-11-12 | - |
dc.date.available | 2010-11-12 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-11 | - |
dc.identifier.citation | BIOTECHNOLOGY TECHNIQUES, v.12, no.11, pp.815 - 818 | - |
dc.identifier.issn | 0951-208X | - |
dc.identifier.uri | http://hdl.handle.net/10203/19800 | - |
dc.description.abstract | Osmotic shock and lysozyme/EDTA methods were used to recover secreted recombinant proteins from protease negative mutant strains of E. coli. Up to 80% of protein A-beta-lactamase fusion protein was recovered from protease negative mutants by simple osmotic shock. Fractionation by lysozyme/EDTA treatment, increased the recovery of protein A-beta-lactamase fusion protein from the mutant strain up to 93%. Mild fractionation condition allowed efficient recovery of secreted protein from protease negative mutant strains, but not from the parent strain possessing proteases. | - |
dc.description.sponsorship | We thank G. Georgiou for providing us with the E. coli strains and plasmid. This study was supported by the academic research fund of Ministry of Education, Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.title | Efficient recovery of secretory recombinant proteins from protease negative mutant Escherichia coli strains | - |
dc.type | Article | - |
dc.identifier.wosid | 000077617600005 | - |
dc.identifier.scopusid | 2-s2.0-0032405508 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 815 | - |
dc.citation.endingpage | 818 | - |
dc.citation.publicationname | BIOTECHNOLOGY TECHNIQUES | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, SJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | protease negative mutant E-coli | - |
dc.subject.keywordAuthor | protein A-beta-lactamase fusion protein | - |
dc.subject.keywordAuthor | osmotic shock | - |
dc.subject.keywordAuthor | lysozyme/EDTA treatment | - |
dc.subject.keywordPlus | PERIPLASM | - |
dc.subject.keywordPlus | RELEASE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.