DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Je-Kyun | - |
dc.contributor.advisor | 박제균 | - |
dc.contributor.author | Lee, Do-Hyun | - |
dc.contributor.author | 이도현 | - |
dc.date.accessioned | 2015-04-23T02:10:21Z | - |
dc.date.available | 2015-04-23T02:10:21Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568476&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/196351 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 바이오및뇌공학과, 2014.2, [ xi, 171 p. ] | - |
dc.description.abstract | This thesis presents an integrated microfluidic platform for interactive single-cell screening of lipid-rich microalgae using droplet microfluidics. To achieve the efficient production of biofuels from algal biomass with economically viable process, selection and growing of highly productive lipid-rich algal strains should be preceded. Especially, the heterogeneity in the lipid accumulation among the individual cells in the same species should be monitored and investigated in a single cell level. Extraordinary advances in droplet microfluidics systems have been made on the basis of the development of droplet manipulation technology with encapsulation method in a single cell level. Monodispersed hydrogel microcapsules can be considered one of the microreactors which encapsulate a cell of interest by utilizing the droplet manipulation technology in a high-throughput manner, and are then transported downstream to allow the quantitative monitor the lipid accumulation of each alga. Also, microcapsules ensure the algal cell viability and capability of other analysis after determination. To guarantee the enhanced single-cell encapsulation efficiency, a new fluid introduction scheme with reduced particle adsorption was first proposed. This idea is based on a novel microparticle injection technique with lateral interconnection, which prevents particle loss, assisted by sample injection along the direction of fluid flow. Biological sample fluids, including fluorescent microparticles, and mammalian (U937) and green algae cells (Chlorella vulgaris), were injected directly via a through-hole drilled in the lateral direction, resulting in a significant reduction in microparticle attachment. It was confirmed that the proposed method accomplished a 100% enhancement of single-cell encapsulation compared to a Poisson distribution. At the high concentration (5×107 cells/mL) of C. vulgaris, the fraction of droplets containing single cells increased gradually from 28.67% to 40.00%... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Hydrogel microcapsule | - |
dc.subject | 단일세포 캡슐화 | - |
dc.subject | 광전자유체역학 | - |
dc.subject | 미세유체역학 | - |
dc.subject | 미세액적 | - |
dc.subject | 미세조류 | - |
dc.subject | Lipid productivity | - |
dc.subject | Microalgae | - |
dc.subject | Microdroplet | - |
dc.subject | Microfluidics | - |
dc.subject | Optoelectrofluidics | - |
dc.subject | Single-cell encapsulation | - |
dc.subject | 하이드로젤 미세캡슐 | - |
dc.subject | 지질 생산 | - |
dc.title | Microdroplet-Based screening of freshwater green algae | - |
dc.title.alternative | 담수 녹조류 선별을 위한 미세액적 기반의 스크리닝 기술 개발 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 568476/325007 | - |
dc.description.department | 한국과학기술원 : 바이오및뇌공학과, | - |
dc.identifier.uid | 020095342 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.localauthor | 박제균 | - |
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