DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Je-Kyun | - |
dc.contributor.advisor | 박제균 | - |
dc.contributor.author | Son, Jae-Jung | - |
dc.contributor.author | 손재정 | - |
dc.date.accessioned | 2015-04-23T02:09:57Z | - |
dc.date.available | 2015-04-23T02:09:57Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568889&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/196321 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2014.2, [ ix, 35 p. ] | - |
dc.description.abstract | We fabricated liver lobule-like stacked hydrogel papers embedded hepatic cells for in vitro liver tissue reconstruction with negligible diffusion limitation. HepG2 cell-embedded mi-cropatterned hydrogel papers which had the width of 8000 μm, the length of 8660 μm and the height of 80-140 μm. The stacked hydrogel papers were fabricated via laminating and aligning the hepatic hydrogel papers in a PDMS jig. For drainage, the jig had many pillars of 170 μm in height. The cells could be cultured in the stacked hydrogel papers which had the height of 300-600 μm exceeding diffusion limit thickness (150-200 μm). The patterned structure with many openings facilitated cell-to-cell interaction and overcame the diffusion limitation, so it gave good viability more than 90% for much remaining HepG2 cells with compared to no pattern. Also, cell-to-cell interaction between each hydrogel paper led to the twofold increase of albumin secretion and the maintenance of urea secretion per million cells with compared to a hydrogel paper. Therefore, we suggest that the stacked hydrogel papers can be macroscale hepatic pseudo-tissues with complex microenvironments, over-coming diffusion limitation for biological study of liver. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Scale-up | - |
dc.subject | Diffusion limitation | - |
dc.subject | 간 조직공학 | - |
dc.subject | 대형화 | - |
dc.subject | Liver tissue engineering | - |
dc.subject | 확산 제약 | - |
dc.title | Reconstruction and characterization of stacked 3D tissue using a hepatic cell-patterned hydrogel paper | - |
dc.title.alternative | 간세포가 패턴된 수화젤 종이를 이용한 적층된 삼차원 조직의 구현 및 특성평가 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 568889/325007 | - |
dc.description.department | 한국과학기술원 : 바이오및뇌공학과, | - |
dc.identifier.uid | 020123354 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.localauthor | 박제균 | - |
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