Structural and biochemical studies on multi-subunit protein complexes: Brucella 26kDa immunogenic protein and chromatin assembly factor-1여러 개의 서브유닛으로 구성된 거대 단백질 복합체(BP26, CAF-1)의 구조 생화학적 연구

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dc.contributor.advisorSong, Ji-Joon-
dc.contributor.advisor송지준-
dc.contributor.authorKim, Dae-Geun-
dc.contributor.author김대근-
dc.date.accessioned2015-04-23T02:08:33Z-
dc.date.available2015-04-23T02:08:33Z-
dc.date.issued2013-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=565543&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/196203-
dc.description학위논문(박사) - 한국과학기술원 : 생명과학과, 2013.8, [ v, 98 p. ]-
dc.description.abstractProteins with tertiary structure assemble into quaternary complexes to carry out their cellular functions. Therefore, to understand the mechanisms of the quaternary complexes, it is important to elucidate their molecular features. In this study, the molecular features of two distinct multi-subunit protein complexes are investigated. First, the crystal structure of Brucella 26kDa Immunogenic protein (BP26) was determined revealing that sixteen molecules of BP26 form a novel channel-like structure. The crystal structure followed by biochemical and electron microscopy (EM) studies indicates that the proteins with SIMPL domain including BP26, LpqG, and IRAK1BP1 generally form multimeric structures. Second, I reconstituted Chromatin Assembly Factor-1 (CAF-1) with Asf1-H3-H4, and investigated the molecular interaction among these proteins by biochemical and EM approaches, delineating the complex formation of CAF-1 and Asf1-H3-H4. This study provides platforms to understand the molecular mechanism of the quaternary protein complexes: BP26 and CAF-1.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectMulti-protein complex-
dc.subject단백질구조-
dc.subject카프원-
dc.subject심플도메인-
dc.subject비피이십육-
dc.subject거대단백질복합체-
dc.subjectBP26-
dc.subjectSIMPL domain-
dc.subjectCAF-1-
dc.subjectProtein structure-
dc.titleStructural and biochemical studies on multi-subunit protein complexes: Brucella 26kDa immunogenic protein and chromatin assembly factor-1-
dc.title.alternative여러 개의 서브유닛으로 구성된 거대 단백질 복합체(BP26, CAF-1)의 구조 생화학적 연구-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN565543/325007 -
dc.description.department한국과학기술원 : 생명과학과, -
dc.identifier.uid020098014-
dc.contributor.localauthorSong, Ji-Joon-
dc.contributor.localauthor송지준-
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