DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yi, Gwan-Su | - |
dc.contributor.advisor | 이관수 | - |
dc.contributor.author | Yi, Shin-Ae | - |
dc.contributor.author | 이신애 | - |
dc.date.accessioned | 2015-04-21T08:41:05Z | - |
dc.date.available | 2015-04-21T08:41:05Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=592334&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/196121 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 2014.8, [ vii, 37 p. ] | - |
dc.description.abstract | Hallmarks of astrocytes include a high resting $K^+$ conductance and a linear current(I)-voltage(V) relationship called passive conductance. The passive conductance is an im-portant driving force for the extracellular ion and neurotransmitter homeostatic function of astrocytes. Recently, the molecular identity of this unique property of astrocytes was identified to be TREK-1/TWIK-1 heterodimers. However, the regulatory mechanism of these channels still remains obscure. Using bioinformatics, the possible regulatory proteins were listed and $Na^+/H^+$ exchanger regulatory factor-1 (NHERF-1) was selected as a novel candidate TWIK-1/TREK-1 heterodimer regulatory protein. NHERF-1 only interacts with the N-terminus region of TREK-1 and the PDZ-domain of NHERF-1 mediates this interaction. When NHERF-1 was overexpressed in cultured astrocytes, TREK-1/TWIK-1 mediated whole cell K+ currents and surface TREK-1/TWIK-1 levels were significantly decreased. On the other hand, knockdown of NHERF-1 in astrocytes using shRNA significantly increased whole cell K+ currents and surface expression of TREK-1/TWIK-1. Taken together, this study reports NHERF-1 regulation of TREK-1/TWIK-1 expression level in astrocytes. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 수동적 전도도 | - |
dc.subject | 트렉-1 | - |
dc.subject | 트윅-1 | - |
dc.subject | 널프-1 | - |
dc.subject | 별아교세포 | - |
dc.subject | K2P channel | - |
dc.subject | K2P 이온통로 | - |
dc.subject | NHERF-1 | - |
dc.subject | TWIK-1 | - |
dc.subject | TREK-1 | - |
dc.subject | Passive conductance | - |
dc.subject | Astrocytes | - |
dc.subject | PDZ domain | - |
dc.subject | PDZ 도메인 | - |
dc.title | NHERF-1 regulating surface expression of K2P channels in astrocytes | - |
dc.title.alternative | NHERF-1에 의한 별아교세포에서의 K2P 이온통로 표면 발현양 조절에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 592334/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020128073 | - |
dc.contributor.localauthor | Yi, Gwan-Su | - |
dc.contributor.localauthor | 이관수 | - |
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