DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Byung Hyun | ko |
dc.contributor.author | Kim, Yong-Tae | ko |
dc.contributor.author | Jung, Jaehwan | ko |
dc.contributor.author | Seo, Tae-Seok | ko |
dc.date.accessioned | 2015-04-06T05:54:30Z | - |
dc.date.available | 2015-04-06T05:54:30Z | - |
dc.date.created | 2014-12-09 | - |
dc.date.created | 2014-12-09 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.citation | MICROCHIMICA ACTA, v.181, no.13-14, pp.1655 - 1668 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | http://hdl.handle.net/10203/194730 | - |
dc.description.abstract | Since the emergence of lab-on-a-chip technology, a variety of chemical and biochemical assays were successfully implemented on microdevice platforms. Among the chip-based applications, genetic analysis based on the polymerase chain reaction (PCR) has been extensively developed in order to accomplish the goal of cheap, rapid, high-throughput, and point-of-care DNA testing. We are summarizing here several formats of the miniaturized PCR systems including the integration of units for sample pretreatment and downstream analytical detection. The various sections cover (a) miniaturized PCR systems, (b) integrated sample pretreatment-PCR microsystems, (c) integrated PCR-detection microsystems, and (d) integrated sample pretreatment-PCR-detection microsystems. Respective microdevices were successfully introduced recently in the form of a fully integrated microsystem for genetic analysis with sample-in-answer-out capability. Contains 120 references. | - |
dc.language | English | - |
dc.publisher | SPRINGER WIEN | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | TOTAL ANALYSIS SYSTEMS | - |
dc.subject | CONTINUOUS-FLOW PCR | - |
dc.subject | CAPILLARY ELECTROPHORESIS MICRODEVICE | - |
dc.subject | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject | ESCHERICHIA-COLI O157H7 | - |
dc.subject | NUCLEIC-ACID ANALYSIS | - |
dc.subject | STEP DNA EXTRACTION | - |
dc.subject | MICROFLUIDIC CHIP | - |
dc.title | Integration of sample pretreatment, μPCR, and detection for a total genetic analysis microsystem | - |
dc.type | Article | - |
dc.identifier.wosid | 000344001000020 | - |
dc.identifier.scopusid | 2-s2.0-84907860935 | - |
dc.type.rims | ART | - |
dc.citation.volume | 181 | - |
dc.citation.issue | 13-14 | - |
dc.citation.beginningpage | 1655 | - |
dc.citation.endingpage | 1668 | - |
dc.citation.publicationname | MICROCHIMICA ACTA | - |
dc.identifier.doi | 10.1007/s00604-013-1128-y | - |
dc.contributor.localauthor | Seo, Tae-Seok | - |
dc.type.journalArticle | Review | - |
dc.subject.keywordAuthor | Lab-on-a-chip | - |
dc.subject.keywordAuthor | Microfluidics | - |
dc.subject.keywordAuthor | Sample-in-answer-out | - |
dc.subject.keywordAuthor | Polymerase chain reaction | - |
dc.subject.keywordAuthor | Sample pretreatment | - |
dc.subject.keywordAuthor | Genetic diagnosis | - |
dc.subject.keywordAuthor | On-site detection | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | TOTAL ANALYSIS SYSTEMS | - |
dc.subject.keywordPlus | CONTINUOUS-FLOW PCR | - |
dc.subject.keywordPlus | CAPILLARY ELECTROPHORESIS MICRODEVICE | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI O157H7 | - |
dc.subject.keywordPlus | NUCLEIC-ACID ANALYSIS | - |
dc.subject.keywordPlus | STEP DNA EXTRACTION | - |
dc.subject.keywordPlus | MICROFLUIDIC CHIP | - |
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