DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rahman, Ziaur | ko |
dc.contributor.author | Sung, Bong Hyun | ko |
dc.contributor.author | Yi, Jiyeun | ko |
dc.contributor.author | Minh, Bui Le | ko |
dc.contributor.author | Lee, Jun-Hyoung | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.date.accessioned | 2015-03-27T07:49:40Z | - |
dc.date.available | 2015-03-27T07:49:40Z | - |
dc.date.created | 2015-01-13 | - |
dc.date.created | 2015-01-13 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.192, pp.187 - 191 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/194478 | - |
dc.description.abstract | Alkanes chemically mimic hydrocarbons found in petroleum, and their demand as biofuels is steadily increasing. Biologically, n-alkanes are produced from fatty acyl-ACPs by acyl-ACP reductases (AARs) and aldehyde deformylating oxygenases (ADOs). One of the major impediments in n-alkane biosynthesis is the low catalytic turnover rates of ADOs. Here, we studied n-alkane biosynthesis in Escherichia coli using a chimeric ADO-AAR fusion protein or zinc finger protein-guided ADO/AAR assembly on DNA scaffolds to control their stoichiometric ratios and spatial arrangements. Bacterial production of n-alkanes with the ADO-AAR fusion protein was increased 4.8-fold (24 mg/L) over a control strain expressing ADO and AAR separately. Optimal n-alkane biosynthesis was achieved when the ADO: AAR binding site ratio on a DNA scaffold was 3: 1, yielding an 8.8-fold increase (44 mg/L) over the control strain. Our findings indicate that the spatial organization of alkane-producing enzymes is critical for efficient n-alkane biosynthesis in E. coli. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | METABOLIC FLUX | - |
dc.subject | EFFICIENCY | - |
dc.title | Enhanced production of n-alkanes in Escherichia coli by spatial organization of biosynthetic pathway enzymes | - |
dc.type | Article | - |
dc.identifier.wosid | 000345970300031 | - |
dc.identifier.scopusid | 2-s2.0-84909944947 | - |
dc.type.rims | ART | - |
dc.citation.volume | 192 | - |
dc.citation.beginningpage | 187 | - |
dc.citation.endingpage | 191 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.jbiotec.2014.10.014 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Sung, Bong Hyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Alkanes | - |
dc.subject.keywordAuthor | Synthetic biology | - |
dc.subject.keywordAuthor | DNA scaffold | - |
dc.subject.keywordAuthor | Chimeric expression | - |
dc.subject.keywordAuthor | Biofuel | - |
dc.subject.keywordPlus | METABOLIC FLUX | - |
dc.subject.keywordPlus | EFFICIENCY | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.