DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Sung-Chul | ko |
dc.contributor.author | Kim, Gahee | ko |
dc.contributor.author | Yang, Hee-Bum | ko |
dc.contributor.author | Park, Kwan Woo | ko |
dc.contributor.author | Kang, Byoung-Cheorl | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2015-01-27T01:43:10Z | - |
dc.date.available | 2015-01-27T01:43:10Z | - |
dc.date.created | 2014-04-28 | - |
dc.date.created | 2014-04-28 | - |
dc.date.created | 2014-04-28 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.54, pp.687 - 694 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/193015 | - |
dc.description.abstract | A novel multiplexing method, which relies on universal amplification of separated ligation-dependent probes (ASLP), has been developed to genotype single-nucleotide polymorphisms (SNPs). The ASLP technique employs two allele-specific oligonucleotides (ASO), modified with universal forward primer sequences at the 5'-end and a common locus-specific oligonucleotide (LSO) extended with a universal separation (US) sequence at the 3'-end. In the process, allele-specific ligation first takes place when target genomic DNA is hybridized by perfectly matching the ASO together with the LSO. A separation probe, which consists of a universal reverse primer sequence labeled with biotin at the 5'-end and complementary sequence of US at the 3'-end, is then applied to the resulting ligation product. During the extension reaction of the separation probe, the ligated probes dissociate from target genomic DNA in the form of a double-stranded DNA and are separated from the reaction mixture, which includes genomic DNA and unligated probes, by simply using streptavidin-coated magnetic beads. PCR amplification of the separated ligation products is then carried out by using universal primers and the PCR products are hybridized on a DNA microarray using the RecA protein. The advantageous features of the new method were demonstrated by using it to genotype 15 SNP markers for cultivar identification of pepper in a convenient and correct manner. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | MOLECULAR INVERSION PROBES | - |
dc.subject | ZIP-CODE MICROARRAY | - |
dc.subject | GOLDENGATE ASSAY | - |
dc.subject | BRCA1 MUTATIONS | - |
dc.subject | PADLOCK PROBES | - |
dc.subject | RECA PROTEIN | - |
dc.subject | DNA | - |
dc.subject | SYSTEM | - |
dc.subject | IDENTIFICATION | - |
dc.subject | HYBRIDIZATION | - |
dc.title | Application of the ASLP technology to a novel platform for rapid and noise-free multiplexed SNP genotyping | - |
dc.type | Article | - |
dc.identifier.wosid | 000333071500103 | - |
dc.identifier.scopusid | 2-s2.0-84890582431 | - |
dc.type.rims | ART | - |
dc.citation.volume | 54 | - |
dc.citation.beginningpage | 687 | - |
dc.citation.endingpage | 694 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2013.10.071 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Kim, Gahee | - |
dc.contributor.nonIdAuthor | Yang, Hee-Bum | - |
dc.contributor.nonIdAuthor | Kang, Byoung-Cheorl | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Amplification of separated ligation-dependent probe | - |
dc.subject.keywordAuthor | ASLP | - |
dc.subject.keywordAuthor | RecA-assisted hybridization | - |
dc.subject.keywordAuthor | Multiplexed SNP genotyping | - |
dc.subject.keywordAuthor | Universal amplification | - |
dc.subject.keywordAuthor | Amplification of separated ligation-dependent probe | - |
dc.subject.keywordAuthor | ASLP | - |
dc.subject.keywordAuthor | RecA-assisted hybridization | - |
dc.subject.keywordAuthor | Multiplexed SNP genotyping | - |
dc.subject.keywordAuthor | Universal amplification | - |
dc.subject.keywordPlus | MOLECULAR INVERSION PROBES | - |
dc.subject.keywordPlus | ZIP-CODE MICROARRAY | - |
dc.subject.keywordPlus | GOLDENGATE ASSAY | - |
dc.subject.keywordPlus | BRCA1 MUTATIONS | - |
dc.subject.keywordPlus | PADLOCK PROBES | - |
dc.subject.keywordPlus | RECA PROTEIN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | MOLECULAR INVERSION PROBES | - |
dc.subject.keywordPlus | ZIP-CODE MICROARRAY | - |
dc.subject.keywordPlus | GOLDENGATE ASSAY | - |
dc.subject.keywordPlus | BRCA1 MUTATIONS | - |
dc.subject.keywordPlus | PADLOCK PROBES | - |
dc.subject.keywordPlus | RECA PROTEIN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
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