Nucleolar GTPase NOG-1 Regulates Development, Fat Storage, and Longevity through Insulin/IGF Signaling in C. elegans

Cited 18 time in webofscience Cited 0 time in scopus
  • Hit : 231
  • Download : 0
NOG1 is a nucleolar GTPase that is critical for 60S ribosome biogenesis. Recently, NOG1 was identified as one of the downstream regulators of target of rapamycin (TOR) in yeast. It is reported that TOR is involved in regulating lifespan and fat storage in Caenorhabditis elegans. Here, we show that the nog1 ortholog (T07A9.9: nog-1) in C. elegans regulates growth, development, lifespan, and fat metabolism. A green fluorescence protein (GFP) promoter assay revealed ubiquitous expression of C. elegans nog-1 from the early embryonic to the adult stage. Furthermore, the GFP-tagged NOG-1 protein is localized to the nucleus, whereas the aberrant NOG-1 protein is concentrated in the nucleolus. Functional studies of NOG-1 in C. elegans further revealed that nog-1 knockdown resulted in smaller broodsize, slower growth, increased life span, and more fat storage. Moreover, nog-1 over-expression resulted in decreased life span. Taken together, our data suggest that nog-1 in C. elegans may be an important player in regulating life span and fat storage via the insulin/IGF pathway.
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Issue Date
2014-01
Language
English
Article Type
Article
Keywords

CAENORHABDITIS-ELEGANS; LIFE-SPAN; SACCHAROMYCES-CEREVISIAE; RIBOSOME BIOGENESIS; BINDING PROTEIN; FUNCTIONAL-CHARACTERIZATION; NUCLEAR EXPORT; CELL-GROWTH; TOR; YEAST

Citation

MOLECULES AND CELLS, v.37, no.1, pp.51 - 57

ISSN
1016-8478
URI
http://hdl.handle.net/10203/192805
Appears in Collection
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 18 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0