DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, Songyi | ko |
dc.contributor.author | Won, Byoung Yeon | ko |
dc.contributor.author | Park, Ki-Soo | ko |
dc.contributor.author | Park, Hyun-Gyu | ko |
dc.date.accessioned | 2014-12-16T01:03:55Z | - |
dc.date.available | 2014-12-16T01:03:55Z | - |
dc.date.created | 2013-09-26 | - |
dc.date.created | 2013-09-26 | - |
dc.date.created | 2013-09-26 | - |
dc.date.issued | 2013-11 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.49, pp.542 - 546 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/192730 | - |
dc.description.abstract | A one-step, electrochemical method for assaying methyltransferase (MTase) activity, based on the convective transport of a quantum dot (QD) signaling tracer, has been developed. The assay chip used in this system was prepared by modifying a gold matrix with CdSe/ZnS QD-tagged dsDNA, which contains a specific methylation site (5'-GATC-3') recognized by MTase. Treatment of the chip with DNA adenine methylation (Dam) MTase, generates a methylated sequence (5'-GAmTC-3') within the dsDNA. The methylated dsDNA is then subjected to a cleavage reaction, induced by DpnI, which leads to release from the gold matrix of a DNA fragment tethered to a QD. Detection of the released QD, using square wave anodic stripping voltammetry (SWASV) on a glassy carbon (GC) electrode, enables the reliable quantitation of the methylated DNA. Because it is accomplished in a simple and convenient one step and does not require any complicated secondary or tedious washing steps, the new assay method holds great promise for epigenetic analysis in facility-limited environments or point-of-care testing (POCT) applications. Crown Copyright (c) 2013 Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | An electrochemical one-step system for assaying methyltransferase activity based on transport of a quantum dot signaling tracer | - |
dc.type | Article | - |
dc.identifier.wosid | 000323396700084 | - |
dc.identifier.scopusid | 2-s2.0-84880638171 | - |
dc.type.rims | ART | - |
dc.citation.volume | 49 | - |
dc.citation.beginningpage | 542 | - |
dc.citation.endingpage | 546 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2013.04.036 | - |
dc.contributor.localauthor | Park, Hyun-Gyu | - |
dc.contributor.nonIdAuthor | Baek, Songyi | - |
dc.contributor.nonIdAuthor | Won, Byoung Yeon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DNA methylation | - |
dc.subject.keywordAuthor | Methyltransferase | - |
dc.subject.keywordAuthor | Epigenetic analysis | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Quantum dot | - |
dc.subject.keywordPlus | DNA METHYLATION | - |
dc.subject.keywordPlus | COLORIMETRIC ASSAY | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | PCR | - |
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