Reversible protein inactivation by optogenetic trapping in cells

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We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2014-06
Language
English
Article Type
Article
Keywords

FLUORESCENT PROTEIN; MAMMALIAN-CELLS; GLOBAL ANALYSIS; BUDDING YEAST; SMALL GTPASES; LIVING CELLS; LIVE CELLS; LOCALIZATION; ACTIVATION; SYSTEM

Citation

NATURE METHODS, v.11, no.6, pp.633 - +

ISSN
1548-7091
DOI
10.1038/NMETH.2940
URI
http://hdl.handle.net/10203/192616
Appears in Collection
BS-Journal Papers(저널논문)
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