DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, S. | ko |
dc.contributor.author | Kim, Pilhan | ko |
dc.contributor.author | Boutilier, R. | ko |
dc.contributor.author | Kim, M. Y. | ko |
dc.contributor.author | Lee, Y. J. | ko |
dc.contributor.author | Lee, H. | ko |
dc.date.accessioned | 2014-11-25T05:33:28Z | - |
dc.date.available | 2014-11-25T05:33:28Z | - |
dc.date.created | 2013-11-05 | - |
dc.date.created | 2013-11-05 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.citation | OPTICS EXPRESS, v.21, no.20, pp.23611 - 23618 | - |
dc.identifier.issn | 1094-4087 | - |
dc.identifier.uri | http://hdl.handle.net/10203/191114 | - |
dc.description.abstract | There has been an increasing interest for observing fast biological phenomena such as cell movements in circulations and action potentials. The laser scanning confocal microscopy offers a good spatial resolution and optical sectioning ability to observe various in vivo animal models. We developed a high speed laser scanning confocal microscope capable of acquiring 512 by 512 pixel images at 200 fps (frames per second). We have incorporated a fast rotating polygonal scanning mirror with 128 facets for the X-axis scanner. In order to increase the throughput of the Y-axis scanner, we applied a bi-directional scanning method for vertical scanning. This made it possible to scan along the Y-axis two times during each scanner motion cycle. For the image acquisition, we used a custom photomultiplier tube amplifier with a broad frequency band. In addition, custom imaging software was written for the new microscope. In order to verify the acquisition speed of the developed confocal microscope, a resolution target moving at a series of constant speeds and a sedated mouse with slight movements due to heartbeats were observed. By comparing successive frames, the frame acquisition speeds were calculated. (C) 2013 Optical Society of America | - |
dc.language | English | - |
dc.publisher | OPTICAL SOC AMER | - |
dc.subject | SYSTEM | - |
dc.subject | CELLS | - |
dc.title | Development of a high speed laser scanning confocal microscope with an acquisition rate up to 200 frames per second | - |
dc.type | Article | - |
dc.identifier.wosid | 000325549800058 | - |
dc.identifier.scopusid | 2-s2.0-84885459934 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 20 | - |
dc.citation.beginningpage | 23611 | - |
dc.citation.endingpage | 23618 | - |
dc.citation.publicationname | OPTICS EXPRESS | - |
dc.identifier.doi | 10.1364/OE.21.023611 | - |
dc.contributor.localauthor | Kim, Pilhan | - |
dc.contributor.nonIdAuthor | Choi, S. | - |
dc.contributor.nonIdAuthor | Boutilier, R. | - |
dc.contributor.nonIdAuthor | Kim, M. Y. | - |
dc.contributor.nonIdAuthor | Lee, Y. J. | - |
dc.contributor.nonIdAuthor | Lee, H. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CELLS | - |
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