DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eunmi | ko |
dc.contributor.author | Lee, Slgirim | ko |
dc.contributor.author | Hong, Seonki | ko |
dc.contributor.author | Jin, Gyuhyung | ko |
dc.contributor.author | Kim, Minhee | ko |
dc.contributor.author | Park, Kook In | ko |
dc.contributor.author | Lee, Haeshin | ko |
dc.contributor.author | Jang, Jae-Hyung | ko |
dc.date.accessioned | 2014-09-01T08:33:05Z | - |
dc.date.available | 2014-09-01T08:33:05Z | - |
dc.date.created | 2014-07-21 | - |
dc.date.created | 2014-07-21 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.citation | ACS APPLIED MATERIALS & INTERFACES, v.6, no.11, pp.8288 - 8294 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189563 | - |
dc.description.abstract | Controlled release of biosuprastructures, such as viruses, from surfaces has been a challenging task in providing efficient ex vivo gene delivery. Conventional controlled viral release approaches have demonstrated low viral immobilization and burst release, inhibiting delivery efficiency. Here, a highly powerful substrate-mediated viral delivery system was designed by combining two key components that have demonstrated great potential in the fields of gene therapy and surface chemistry, respectively: adeno-associated viral (AAV) vectors and adhesive catecholamine surfaces. The introduction of a nanoscale thin coating of catecholamines, poly(norepinephrine) (pNE) or poly(dopamine) (pDA) to provide AAV adhesion followed by human neural stem cell (hNSC) culture on sticky solid surfaces exhibited unprecedented results: approximately 90% loading vs 25% (AAV_bare surface), no burst release, sustained release at constant rates, approximately 70% infection vs 20% (AAV_bare surface), and rapid internalization. Importantly, the sticky catecholamine-mediated AAV delivery system successfully induced a physiological response from hNSCs, cellular proliferation by a single-shot of AAV encoding fibroblast growth factor-2 (FGF-2), which is typically achieved by multiple treatments with expensive FGF-2 proteins. By combining the adhesive material-independent surface functionalization characters of pNE and pDA, this new sticky "delivering-from" gene delivery platform will make a significant contribution to numerous fields, including tissue engineering, gene therapy, and stem cell therapy. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | TISSUE ENGINEERING SCAFFOLDS | - |
dc.subject | LOCALIZED GENE DELIVERY | - |
dc.subject | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject | SURFACE-CHEMISTRY | - |
dc.subject | DNA DELIVERY | - |
dc.subject | POLYDOPAMINE | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | ADSORPTION | - |
dc.subject | RELEASE | - |
dc.subject | ROUTE | - |
dc.title | Sticky "Delivering-From" Strategies Using Viral Vectors for Efficient Human Neural Stem Cell Infection by Bioinspired Catecholamines | - |
dc.type | Article | - |
dc.identifier.wosid | 000337336900040 | - |
dc.identifier.scopusid | 2-s2.0-84902438570 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 8288 | - |
dc.citation.endingpage | 8294 | - |
dc.citation.publicationname | ACS APPLIED MATERIALS & INTERFACES | - |
dc.identifier.doi | 10.1021/am5011095 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Haeshin | - |
dc.contributor.nonIdAuthor | Kim, Eunmi | - |
dc.contributor.nonIdAuthor | Lee, Slgirim | - |
dc.contributor.nonIdAuthor | Jin, Gyuhyung | - |
dc.contributor.nonIdAuthor | Kim, Minhee | - |
dc.contributor.nonIdAuthor | Park, Kook In | - |
dc.contributor.nonIdAuthor | Jang, Jae-Hyung | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | substrate-mediated gene delivery | - |
dc.subject.keywordAuthor | adeno-associated virus | - |
dc.subject.keywordAuthor | poly(norepinephrine) | - |
dc.subject.keywordAuthor | poly(dopamine) | - |
dc.subject.keywordAuthor | neural stem cells | - |
dc.subject.keywordAuthor | sticky interfaces | - |
dc.subject.keywordPlus | TISSUE ENGINEERING SCAFFOLDS | - |
dc.subject.keywordPlus | LOCALIZED GENE DELIVERY | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | SURFACE-CHEMISTRY | - |
dc.subject.keywordPlus | DNA DELIVERY | - |
dc.subject.keywordPlus | POLYDOPAMINE | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | ROUTE | - |
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