Identification and Characterization of a Ginsenoside-Transforming beta-Glucosidase from Pseudonocardia sp Gsoil 1536 and Its Application for Enhanced Production of Minor Ginsenoside Rg(2)(S)

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dc.contributor.authorDu, Juanko
dc.contributor.authorCUI, CHANGHAOko
dc.contributor.authorPark, Sung Chulko
dc.contributor.authorKim, Jin-Kwangko
dc.contributor.authorYu, Hong-Shanko
dc.contributor.authorJin, Feng-Xieko
dc.contributor.authorSun, Changkaiko
dc.contributor.authorKim, Sun-Changko
dc.contributor.authorIm, Wan-Taekko
dc.date.accessioned2014-09-01T08:08:22Z-
dc.date.available2014-09-01T08:08:22Z-
dc.date.created2014-07-17-
dc.date.created2014-07-17-
dc.date.issued2014-06-
dc.identifier.citationPLOS ONE, v.9, no.6-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10203/189407-
dc.description.abstractThe ginsenoside Rg(2)(S), which is one of the pharmaceutical components of ginseng, is known to have neuroprotective, antiinflammation, and anti-diabetic effects. However, the usage of ginsenoside Rg2(S) is restricted owing to the small amounts found in white and red ginseng. To enhance the production of ginsenoside Rg2(S) as a 100 gram unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the recombinant glycoside hydrolase (BglPC28), which is a ginsenoside-transforming recombinant beta-glucosidase from Pseudonocardia sp. strain Gsoil 1536. The gene, termed bglPC28, encoding beta-glucosidase (BglPC28) belonging to the glycoside hydrolase family 3 was cloned. bglPC28 consists of 2,232 bp (743 amino acid residues) with a predicted molecular mass of 78,975 Da. This enzyme was overexpressed in Escherichia coli BL21(DE3) using a GST-fused pGEX 4T-1 vector system. The optimum conditions of the recombinant BglPC28 were pH 7.0 and 37 degrees C. BglPC28 can effectively transform the ginsenoside Re to Rg2(S); the Km values of PNPG and Re were 6.36 +/- 1.10 and 1.42 +/- 0.13 mM, respectively, and the V-max values were 40.0 +/- 2.55 and 5.62 +/- 0.21 mmol min(-1) mg(-)1 of protein, respectively. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 7.0 and 30 degrees C for 12 hours with a concentration of 20 mg/ml of ginsenoside Re from American ginseng roots. Finally, 113 g of Rg2(S) was produced from 150 g of Re with 84.0 +/- 1.1% chromatographic purity. These results suggest that this enzymatic method could be usefully exploited in the preparation of ginsenoside Rg(2)(S) in the cosmetics, functional food, and pharmaceutical industries.-
dc.languageEnglish-
dc.publisherPUBLIC LIBRARY SCIENCE-
dc.subjectALPHA-L-ARABINOFURANOSIDASE-
dc.subjectPANAX-GINSENG-
dc.subjectCOMPOUND K-
dc.subjectENZYME CHARACTERIZATION-
dc.subjectMICROBACTERIUM-ESTERAROMATICUM-
dc.subjectSIMULTANEOUS QUANTIFICATION-
dc.subjectGLYCOSIDE HYDROLASES-
dc.subjectCONVERTING ACTIVITY-
dc.subjectRARE GINSENOSIDES-
dc.subjectPA14 DOMAIN-
dc.titleIdentification and Characterization of a Ginsenoside-Transforming beta-Glucosidase from Pseudonocardia sp Gsoil 1536 and Its Application for Enhanced Production of Minor Ginsenoside Rg(2)(S)-
dc.typeArticle-
dc.identifier.wosid000337165600123-
dc.identifier.scopusid2-s2.0-84902665729-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.issue6-
dc.citation.publicationnamePLOS ONE-
dc.identifier.doi10.1371/journal.pone.0096914-
dc.contributor.localauthorKim, Sun-Chang-
dc.contributor.nonIdAuthorDu, Juan-
dc.contributor.nonIdAuthorPark, Sung Chul-
dc.contributor.nonIdAuthorKim, Jin-Kwang-
dc.contributor.nonIdAuthorYu, Hong-Shan-
dc.contributor.nonIdAuthorJin, Feng-Xie-
dc.contributor.nonIdAuthorSun, Changkai-
dc.contributor.nonIdAuthorIm, Wan-Taek-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusALPHA-L-ARABINOFURANOSIDASE-
dc.subject.keywordPlusPANAX-GINSENG-
dc.subject.keywordPlusCOMPOUND K-
dc.subject.keywordPlusENZYME CHARACTERIZATION-
dc.subject.keywordPlusMICROBACTERIUM-ESTERAROMATICUM-
dc.subject.keywordPlusSIMULTANEOUS QUANTIFICATION-
dc.subject.keywordPlusGLYCOSIDE HYDROLASES-
dc.subject.keywordPlusCONVERTING ACTIVITY-
dc.subject.keywordPlusRARE GINSENOSIDES-
dc.subject.keywordPlusPA14 DOMAIN-
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