DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Won Chul | ko |
dc.contributor.author | Pisano, Albert P. | ko |
dc.contributor.author | Cho, Young-Ho | ko |
dc.contributor.author | Kuypers, Frans A. | ko |
dc.date.accessioned | 2014-09-01T07:58:40Z | - |
dc.date.available | 2014-09-01T07:58:40Z | - |
dc.date.created | 2013-08-26 | - |
dc.date.created | 2013-08-26 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | MICRO & NANO LETTERS, v.8, no.4, pp.169 - 172 | - |
dc.identifier.issn | 1750-0443 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189378 | - |
dc.description.abstract | Presented is a single-cell analyser to measure cytoplasmic components in large cell populations. This technology, flow-lysometry, uses a flow-cell that performs three functions (cell detection, cell lysis and component sensing) in a continuous microfluidic channel. This flow-cell can be coupled and synchronised with classical flowcytometry, thus offering high-throughput measurements of various cytoplasmic compounds in combination with cell-surface markers. The measurement of cytoplasmic markers in the cytosol of red blood cells was shown to verify population analysis of individual cells in mixed-cell populations. This work presents an approach to characterise complex cell populations to extend measurements in cell biology. | - |
dc.language | English | - |
dc.publisher | INST ENGINEERING TECHNOLOGY-IET | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | LYSIS DEVICE | - |
dc.subject | CYTOMETRY | - |
dc.subject | PROCESSOR | - |
dc.subject | PROTEINS | - |
dc.subject | CHIP | - |
dc.title | Flow-lysometry for cytoplasmic analysis of single cells in large populations | - |
dc.type | Article | - |
dc.identifier.wosid | 000321858100001 | - |
dc.identifier.scopusid | 2-s2.0-84879342148 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 169 | - |
dc.citation.endingpage | 172 | - |
dc.citation.publicationname | MICRO & NANO LETTERS | - |
dc.identifier.doi | 10.1049/mnl.2012.0722 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Cho, Young-Ho | - |
dc.contributor.nonIdAuthor | Lee, Won Chul | - |
dc.contributor.nonIdAuthor | Pisano, Albert P. | - |
dc.contributor.nonIdAuthor | Kuypers, Frans A. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | LYSIS DEVICE | - |
dc.subject.keywordPlus | CYTOMETRY | - |
dc.subject.keywordPlus | PROCESSOR | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CHIP | - |
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