DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Jung Eun | ko |
dc.contributor.author | Hur, Wonhee | ko |
dc.contributor.author | Kim, Jung-Hee | ko |
dc.contributor.author | Li, Tian Zhu | ko |
dc.contributor.author | Lee, Eun Byul | ko |
dc.contributor.author | Lee, Sung Won | ko |
dc.contributor.author | Kang, Won-Seok | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Wakita, Takaji | ko |
dc.contributor.author | Yoon, Seung Kew | ko |
dc.date.accessioned | 2014-09-01T07:39:11Z | - |
dc.date.available | 2014-09-01T07:39:11Z | - |
dc.date.created | 2014-06-30 | - |
dc.date.created | 2014-06-30 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.citation | PLOS ONE, v.9, no.5 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189301 | - |
dc.description.abstract | Background and Aims: Despite the discovery of hepatitis C virus (HCV) entry factor, the mechanism by which it is regulated by miRNAs remains unclear. Adipose tissue-derived human mesenchymal stem cells (AT-hMSCs) have been widely used for differentiated hepatocyte-like cells (DHCs). Here, we established an in vitro HCV infection model using DHCs from AT-hMSCs and identified miRNAs that modulate HCV infectivity. Methods: AT-hMSCs were differentiated into DHCs using the conditional media, and evaluated for hepatocyte characteristics using RT-PCR, immunocytochemistry, periodic acid-Schiff staining, and a urea synthesis assay. The expression of HCV candidate receptors was also verified using immunocytochemistry. The levels of candidate miRNAs targeting HCV receptors were then determined by relative quantitative RT-PCR (rqRT-PCR). Finally, DHCs were infected using HCVcc and serum from HCV-infected patients, and infectivity of the virus was measured by rqRT-PCR and transmission electron microscopy (TEM). Results: The expected changes in morphology, function and hepatic gene expression were observed during hepatic differentiation. Moreover, the expression of candidate HCV entry factors and miR-27a were altered during hepatic differentiation. The infection and replication of HCV occurred efficiently in DHCs treated with HCVcc or infected with serum from HCV-infected patients. In addition, HCV infectivity was suppressed in miR-27a-transfected DHCs, due to the inhibition of LDLR expression by miR-27a. Conclusions: Our results demonstrate that AT-hMSCs are a good source of DHCs, which are suitable for in vitro cultivation of HCV. Furthermore, these results suggest that miR-27a modulates HCV infectivity by regulating LDLR expression. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | HEPATITIS-C VIRUS | - |
dc.subject | EXPRESSION | - |
dc.subject | REPLICATION | - |
dc.subject | GENERATION | - |
dc.subject | SUPPORT | - |
dc.title | MicroRNA-27a Modulates HCV Infection in Differentiated Hepatocyte-Like Cells from Adipose Tissue-Derived Mesenchymal Stem Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000336369200001 | - |
dc.identifier.scopusid | 2-s2.0-84901326993 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 5 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0091958 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Choi, Jung Eun | - |
dc.contributor.nonIdAuthor | Hur, Wonhee | - |
dc.contributor.nonIdAuthor | Kim, Jung-Hee | - |
dc.contributor.nonIdAuthor | Li, Tian Zhu | - |
dc.contributor.nonIdAuthor | Lee, Eun Byul | - |
dc.contributor.nonIdAuthor | Lee, Sung Won | - |
dc.contributor.nonIdAuthor | Wakita, Takaji | - |
dc.contributor.nonIdAuthor | Yoon, Seung Kew | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | HEPATITIS-C VIRUS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | SUPPORT | - |
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