Leucine-rich repeat-containing G-protein coupled receptor 5/GPR49 activates G(12/13)-Rho GTPase pathway

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dc.contributor.authorKwon, Mi Soko
dc.contributor.authorPark, Bi-ohko
dc.contributor.authorKim, Ho Minko
dc.contributor.authorKim, Sunhongko
dc.date.accessioned2014-08-29T02:15:52Z-
dc.date.available2014-08-29T02:15:52Z-
dc.date.created2013-10-22-
dc.date.created2013-10-22-
dc.date.issued2013-09-
dc.identifier.citationMOLECULES AND CELLS, v.36, no.3, pp.267 - 272-
dc.identifier.issn1016-8478-
dc.identifier.urihttp://hdl.handle.net/10203/189006-
dc.description.abstractLeucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is highly expressed in adult stem cells of various tissues, such as intestine, hair follicles, and stomach. LGR5 is also overexpressed in some colon and ovarian tumors. Recent reports show that R-spondin (RSPO) family ligands bind to and activate LGR5, enhancing canonical Wnt signaling via the interaction with LRP5/6 and Frizzled. The identity of heterotrimeric G-proteins coupled to LGR5, however, remains unclear. Here, we show that Rho GTPase is a downstream target of LGR5. Overexpression of LGR5 induced SRF-RE luciferase activity, a reporter of Rho signaling. RSPOs, ligands for LGR4, LGR5, and LGR6, however, did not induce SRF-RE reporter activity in the presence of LGR5. Consistently, LGR5-induced activity of the SRF-RE reporter was inhibited by Rho inhibitor C3 transferase and RhoA N19 mutant, and knockdown of G alpha(12/13) genes blocked the reporter activity induced by LGR5. In addition, focal adhesion kinase, NF-kappa B and c-fos, targets of Rho GTPase, were shown to be regulated by LGR5. Here, we have demonstrated, for the first time, that LGR5 is coupled to the Rho pathway through G(12/13) signaling.-
dc.languageEnglish-
dc.publisherKOREAN SOC MOLECULAR & CELLULAR BIOLOGY-
dc.subjectNF-KAPPA-B-
dc.subjectFOCAL ADHESION KINASE-
dc.subjectRHO-GTPASES-
dc.subjectWNT/BETA-CATENIN-
dc.subjectSTEM-CELLS-
dc.subjectTYROSINE PHOSPHORYLATION-
dc.subjectSIGNALING PATHWAYS-
dc.subjectWNT RECEPTORS-
dc.subjectR-SPONDINS-
dc.subjectNULL MICE-
dc.titleLeucine-rich repeat-containing G-protein coupled receptor 5/GPR49 activates G(12/13)-Rho GTPase pathway-
dc.typeArticle-
dc.identifier.wosid000324643100011-
dc.identifier.scopusid2-s2.0-84893005204-
dc.type.rimsART-
dc.citation.volume36-
dc.citation.issue3-
dc.citation.beginningpage267-
dc.citation.endingpage272-
dc.citation.publicationnameMOLECULES AND CELLS-
dc.identifier.doi10.1007/s10059-013-0173-z-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorKim, Ho Min-
dc.contributor.nonIdAuthorKwon, Mi So-
dc.contributor.nonIdAuthorPark, Bi-oh-
dc.contributor.nonIdAuthorKim, Sunhong-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorFAK-
dc.subject.keywordAuthorG(12/13)-
dc.subject.keywordAuthorLGR5-
dc.subject.keywordAuthorNFkappaB-
dc.subject.keywordAuthorRho-
dc.subject.keywordPlusNF-KAPPA-B-
dc.subject.keywordPlusFOCAL ADHESION KINASE-
dc.subject.keywordPlusRHO-GTPASES-
dc.subject.keywordPlusWNT/BETA-CATENIN-
dc.subject.keywordPlusSTEM-CELLS-
dc.subject.keywordPlusTYROSINE PHOSPHORYLATION-
dc.subject.keywordPlusSIGNALING PATHWAYS-
dc.subject.keywordPlusWNT RECEPTORS-
dc.subject.keywordPlusR-SPONDINS-
dc.subject.keywordPlusNULL MICE-
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