DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Mi So | ko |
dc.contributor.author | Park, Bi-oh | ko |
dc.contributor.author | Kim, Ho Min | ko |
dc.contributor.author | Kim, Sunhong | ko |
dc.date.accessioned | 2014-08-29T02:15:52Z | - |
dc.date.available | 2014-08-29T02:15:52Z | - |
dc.date.created | 2013-10-22 | - |
dc.date.created | 2013-10-22 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.36, no.3, pp.267 - 272 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189006 | - |
dc.description.abstract | Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is highly expressed in adult stem cells of various tissues, such as intestine, hair follicles, and stomach. LGR5 is also overexpressed in some colon and ovarian tumors. Recent reports show that R-spondin (RSPO) family ligands bind to and activate LGR5, enhancing canonical Wnt signaling via the interaction with LRP5/6 and Frizzled. The identity of heterotrimeric G-proteins coupled to LGR5, however, remains unclear. Here, we show that Rho GTPase is a downstream target of LGR5. Overexpression of LGR5 induced SRF-RE luciferase activity, a reporter of Rho signaling. RSPOs, ligands for LGR4, LGR5, and LGR6, however, did not induce SRF-RE reporter activity in the presence of LGR5. Consistently, LGR5-induced activity of the SRF-RE reporter was inhibited by Rho inhibitor C3 transferase and RhoA N19 mutant, and knockdown of G alpha(12/13) genes blocked the reporter activity induced by LGR5. In addition, focal adhesion kinase, NF-kappa B and c-fos, targets of Rho GTPase, were shown to be regulated by LGR5. Here, we have demonstrated, for the first time, that LGR5 is coupled to the Rho pathway through G(12/13) signaling. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | FOCAL ADHESION KINASE | - |
dc.subject | RHO-GTPASES | - |
dc.subject | WNT/BETA-CATENIN | - |
dc.subject | STEM-CELLS | - |
dc.subject | TYROSINE PHOSPHORYLATION | - |
dc.subject | SIGNALING PATHWAYS | - |
dc.subject | WNT RECEPTORS | - |
dc.subject | R-SPONDINS | - |
dc.subject | NULL MICE | - |
dc.title | Leucine-rich repeat-containing G-protein coupled receptor 5/GPR49 activates G(12/13)-Rho GTPase pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000324643100011 | - |
dc.identifier.scopusid | 2-s2.0-84893005204 | - |
dc.type.rims | ART | - |
dc.citation.volume | 36 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 267 | - |
dc.citation.endingpage | 272 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.identifier.doi | 10.1007/s10059-013-0173-z | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Ho Min | - |
dc.contributor.nonIdAuthor | Kwon, Mi So | - |
dc.contributor.nonIdAuthor | Park, Bi-oh | - |
dc.contributor.nonIdAuthor | Kim, Sunhong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | FAK | - |
dc.subject.keywordAuthor | G(12/13) | - |
dc.subject.keywordAuthor | LGR5 | - |
dc.subject.keywordAuthor | NFkappaB | - |
dc.subject.keywordAuthor | Rho | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | FOCAL ADHESION KINASE | - |
dc.subject.keywordPlus | RHO-GTPASES | - |
dc.subject.keywordPlus | WNT/BETA-CATENIN | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | WNT RECEPTORS | - |
dc.subject.keywordPlus | R-SPONDINS | - |
dc.subject.keywordPlus | NULL MICE | - |
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