DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, Yong Hwang | ko |
dc.contributor.author | Yang, Aerin | ko |
dc.contributor.author | Lee, Seyoung | ko |
dc.contributor.author | Kim, Kibong | ko |
dc.contributor.author | Liew, Hyunjeong | ko |
dc.contributor.author | Lee, Sang Hyung | ko |
dc.contributor.author | Lee, Ju Eun | ko |
dc.contributor.author | Lee, Hong-In | ko |
dc.contributor.author | Suh, Yoo-Hun | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.contributor.author | Churchill, David G | ko |
dc.date.accessioned | 2014-08-29T01:08:38Z | - |
dc.date.available | 2014-08-29T01:08:38Z | - |
dc.date.created | 2014-02-10 | - |
dc.date.created | 2014-02-10 | - |
dc.date.issued | 2014-03 | - |
dc.identifier.citation | JOURNAL OF NEUROSCIENCE RESEARCH, v.92, no.3, pp.359 - 368 | - |
dc.identifier.issn | 0360-4012 | - |
dc.identifier.uri | http://hdl.handle.net/10203/188695 | - |
dc.description.abstract | alpha-Synuclein oligomers can induce neurotoxicity and are implicated in Parkinson's disease etiology and disease progression. Many studies have reported alpha-synuclein oligomerization by dopamine (DA) and transition metal ions, but few studies provide insight into joint influences of DA and Cu2+. In this study, DA and Cu2+ were coadministered aerobically to measure alpha-synuclein oligomerization under these conditions. In the presence of oxygen, DA induced alpha-synuclein oligomerization in a dose-dependent manner. Cu+/2+ did not effect oligomerization in such a manner in the presence of DA. By electrophoresis, Cu2+ was found easily to induce oligomerization with DA. This implies that oligomerization invoked by DA is reversible in the presence of Cu2+, which appears to be mediated by noncovalent bond interactions. In the absence of oxygen, DA induced less oligomerization of alpha-synuclein, whereas DA/Cu2+ induced aerobic-level amounts of oligomers, suggesting that DA/Cu2+ induces oligomerization independent of oxygen concentration. Radical species were detected through electron paramagnetic resonance (EPR) spectroscopic analysis arising from coincubation of DA/Cu2+ with alpha-synuclein. Redox reactions induced by DA/Cu2+ were observed in multimer regions of alpha-synuclein oligomers through NBT assay. Cellular toxicity results confirm that, for normal and hypoxic conditions, copper or DA/Cu2+ can induce cell death, which may arise from copper redox chemistry. From these results, we propose that DA and DA/Cu2+ induce different mechanisms of alpha-synuclein oligomerization, cross-linking with noncovalent (or reversible covalent) bonding vs. likely radical-mediated covalent modification. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | METAL-CATALYZED OXIDATION | - |
dc.subject | PARKINSONS-DISEASE | - |
dc.subject | AGGREGATION | - |
dc.subject | BINDING | - |
dc.subject | NEUROTOXICITY | - |
dc.subject | PHOSPHOSERINE | - |
dc.subject | COPPER(II) | - |
dc.subject | METABOLITE | - |
dc.subject | PROTEINS | - |
dc.subject | CATECHOL | - |
dc.title | Dopamine and Cu+/(2+) Can Induce Oligomerization of alpha-Synuclein in the Absence of Oxygen: Two Types of Oligomerization Mechanisms for alpha-Synuclein and Related Cell Toxicity Studies | - |
dc.type | Article | - |
dc.identifier.wosid | 000329677000009 | - |
dc.identifier.scopusid | 2-s2.0-84892484610 | - |
dc.type.rims | ART | - |
dc.citation.volume | 92 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 359 | - |
dc.citation.endingpage | 368 | - |
dc.citation.publicationname | JOURNAL OF NEUROSCIENCE RESEARCH | - |
dc.identifier.doi | 10.1002/jnr.23323 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.localauthor | Churchill, David G | - |
dc.contributor.nonIdAuthor | Lee, Seyoung | - |
dc.contributor.nonIdAuthor | Liew, Hyunjeong | - |
dc.contributor.nonIdAuthor | Lee, Sang Hyung | - |
dc.contributor.nonIdAuthor | Lee, Ju Eun | - |
dc.contributor.nonIdAuthor | Lee, Hong-In | - |
dc.contributor.nonIdAuthor | Suh, Yoo-Hun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | alpha-synuclein | - |
dc.subject.keywordAuthor | dopamine | - |
dc.subject.keywordAuthor | copper | - |
dc.subject.keywordAuthor | protein cross-linking | - |
dc.subject.keywordAuthor | radical-mediated chemistry | - |
dc.subject.keywordPlus | METAL-CATALYZED OXIDATION | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | NEUROTOXICITY | - |
dc.subject.keywordPlus | PHOSPHOSERINE | - |
dc.subject.keywordPlus | COPPER(II) | - |
dc.subject.keywordPlus | METABOLITE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CATECHOL | - |
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