DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Karam | ko |
dc.contributor.author | Yang, Jinhee | ko |
dc.contributor.author | Zhong, Xiao-Ping | ko |
dc.contributor.author | Kim, Myoung-Hwan | ko |
dc.contributor.author | Kim, Yun Sook | ko |
dc.contributor.author | Lee, Hyun Woo | ko |
dc.contributor.author | Han, Seungnam | ko |
dc.contributor.author | Choi, Jeonghoon | ko |
dc.contributor.author | Han, Kihoon | ko |
dc.contributor.author | Seo, Jinsoo | ko |
dc.contributor.author | Prescott, Stephen M. | ko |
dc.contributor.author | Topham, Matthew K. | ko |
dc.contributor.author | Bae, Yong Chul | ko |
dc.contributor.author | Koretzky, Gary | ko |
dc.contributor.author | Choi, Se-Young | ko |
dc.contributor.author | Kim, Eunjoon | ko |
dc.date.accessioned | 2010-05-27T05:40:13Z | - |
dc.date.available | 2010-05-27T05:40:13Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | EMBO JOURNAL, v.28, no.8, pp.1170 - 1179 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.uri | http://hdl.handle.net/10203/18590 | - |
dc.description.abstract | Diacylglycerol (DAG) is an important lipid signalling molecule that exerts an effect on various effector proteins including protein kinase C. A main mechanism for DAG removal is to convert it to phosphatidic acid (PA) by DAG kinases (DGKs). However, it is not well understood how DGKs are targeted to specific subcellular sites and tightly regulates DAG levels. The neuronal synapse is a prominent site of DAG production. Here, we show that DGK zeta is targeted to excitatory synapses through its direct interaction with the postsynaptic PDZ scaffold PSD-95. Overexpression of DGK zeta in cultured neurons increases the number of dendritic spines, which receive the majority of excitatory synaptic inputs, in a manner requiring its catalytic activity and PSD-95 binding. Conversely, DGK zeta knockdown reduces spine density. Mice deficient in DGK zeta expression show reduced spine density and excitatory synaptic transmission. Time-lapse imaging indicates that DGK zeta is required for spine maintenance but not formation. We propose that PSD-95 targets DGK zeta to synaptic DAG-producing receptors to tightly couple synaptic DAG production to its conversion to PA for the maintenance of spine density. | - |
dc.description.sponsorship | This study was supported by the National Creative Research Initiative Program of the Korean Ministry of Science and Technology (to EK), the Korea Research Foundation Grant (KRF-2006-312-C00360; to S-YC), the MRC Program of the Korea Science and Engineering Foundation (KOSEF: R13-2008-009-01001-0; to YCB), the United States National Institutes of Health (R01-CA95463; to MKT), and Huntsman Cancer Foundation (to MKT). We thank Dr Christopher Nelson for the help with the PA assay. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | LONG-TERM DEPRESSION | - |
dc.subject | MAGUK SCAFFOLDING PROTEINS | - |
dc.subject | POSTSYNAPTIC DENSITY | - |
dc.subject | PHOSPHOLIPASE-C | - |
dc.subject | AMPA RECEPTORS | - |
dc.subject | MOLECULAR-MECHANISMS | - |
dc.subject | GLUTAMATE RECEPTORS | - |
dc.subject | EXCITATORY SYNAPSES | - |
dc.subject | ACTIN CYTOSKELETON | - |
dc.subject | GUANYLATE KINASES | - |
dc.title | Synaptic removal of diacylglycerol by DGK zeta and PSD-95 regulates dendritic spine maintenance | - |
dc.type | Article | - |
dc.identifier.wosid | 000265478400018 | - |
dc.identifier.scopusid | 2-s2.0-67349239306 | - |
dc.type.rims | ART | - |
dc.citation.volume | 28 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1170 | - |
dc.citation.endingpage | 1179 | - |
dc.citation.publicationname | EMBO JOURNAL | - |
dc.identifier.doi | 10.1038/emboj.2009.44 | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Kim, Karam | - |
dc.contributor.nonIdAuthor | Zhong, Xiao-Ping | - |
dc.contributor.nonIdAuthor | Kim, Myoung-Hwan | - |
dc.contributor.nonIdAuthor | Kim, Yun Sook | - |
dc.contributor.nonIdAuthor | Lee, Hyun Woo | - |
dc.contributor.nonIdAuthor | Seo, Jinsoo | - |
dc.contributor.nonIdAuthor | Prescott, Stephen M. | - |
dc.contributor.nonIdAuthor | Topham, Matthew K. | - |
dc.contributor.nonIdAuthor | Bae, Yong Chul | - |
dc.contributor.nonIdAuthor | Koretzky, Gary | - |
dc.contributor.nonIdAuthor | Choi, Se-Young | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DGK zeta | - |
dc.subject.keywordAuthor | diacylglycerol kinase | - |
dc.subject.keywordAuthor | phosphatidic acid | - |
dc.subject.keywordAuthor | PSD-95 | - |
dc.subject.keywordAuthor | spine | - |
dc.subject.keywordPlus | LONG-TERM DEPRESSION | - |
dc.subject.keywordPlus | MAGUK SCAFFOLDING PROTEINS | - |
dc.subject.keywordPlus | POSTSYNAPTIC DENSITY | - |
dc.subject.keywordPlus | PHOSPHOLIPASE-C | - |
dc.subject.keywordPlus | AMPA RECEPTORS | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | GLUTAMATE RECEPTORS | - |
dc.subject.keywordPlus | EXCITATORY SYNAPSES | - |
dc.subject.keywordPlus | ACTIN CYTOSKELETON | - |
dc.subject.keywordPlus | GUANYLATE KINASES | - |
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