DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Min-Kyu | ko |
dc.contributor.author | Seo, Moon-Hyeong | ko |
dc.contributor.author | Lee, Do-Hoon | ko |
dc.contributor.author | Kim, Seung-Hyun | ko |
dc.contributor.author | Kim, Hae-Sim | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2010-05-10T07:14:47Z | - |
dc.date.available | 2010-05-10T07:14:47Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.citation | ANALYTICAL SCIENCES, v.23, no.5, pp.545 - 549 | - |
dc.identifier.issn | 0910-6340 | - |
dc.identifier.uri | http://hdl.handle.net/10203/18156 | - |
dc.description.abstract | A sensitive and multiplexed assay of allergen-specific human immunoglobulin E (IgE) is of great significance in the precise diagnosis of allergies. We report on the optimization of critical factors for chip-based analysis of IgE in human serum with a high reliability. Extracts of two mite species were used as model allergens, and were spotted onto a glass slide for the construction of an allergen chip. Respective allergen-specific IgE in human serum was analyzed by using biotinylated anti-human IgE and a streptavidin-Cy3 conjugate. Factors affecting the performance of the allergen chip were investigated and optimized. Especially, the effect of additives, the concentrations of biotinylated anti-human IgE and the streptavidin-Cy3 conjugate, the serum dilution factor, and the concentration of allergen extract as a capturing agent were examined in detail. Under the optimized conditions, a chip-based analysis for sera from 43 patients revealed a reliable and reproducible diagnosis of respective allergies, showing a good correlation with a conventional CAP assay. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | JAPAN SOC ANALYTICAL CHEMISTRY | - |
dc.subject | RECOMBINANT ALLERGENS | - |
dc.subject | IMMUNOGLOBULIN-E | - |
dc.subject | MICROARRAYS | - |
dc.title | Optimization of critical factors affecting the performance of an allergen chip for the analysis of an allergen-specific human IgE in serum | - |
dc.type | Article | - |
dc.identifier.wosid | 000246716000008 | - |
dc.identifier.scopusid | 2-s2.0-34447310056 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 545 | - |
dc.citation.endingpage | 549 | - |
dc.citation.publicationname | ANALYTICAL SCIENCES | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Han, Min-Kyu | - |
dc.contributor.nonIdAuthor | Lee, Do-Hoon | - |
dc.contributor.nonIdAuthor | Kim, Seung-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Hae-Sim | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RECOMBINANT ALLERGENS | - |
dc.subject.keywordPlus | IMMUNOGLOBULIN-E | - |
dc.subject.keywordPlus | MICROARRAYS | - |
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