Synthesis of gold-coated magnetic microparticles and their application for electrochemical glucose sensing by the enzymatically precipitated prussian blue

Abstract

An enzyme stimulated deposition of Prussian blue (PB) onto gold-coated magnetic microparticles (MMPs) is described. We synthesize a continuous outer gold layer on MMPs for a gold working electrode and evaluate its superparamagnetic properties. The gold-coated MMPs offered an adhesive layer for the immobilization of glucose oxidase, catalyzing the generation of PB in the presence of glucose. The assembled PB on the gold shell surfaces was detected with electrochemical measurements. Recording a linear response from 0.2 to 10 mM of glucose, the applicability of magnetic core-gold shell structures to an electrochemical bioassay has been successfully demonstrated.