DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Koh, Gou-Young | - |
dc.contributor.advisor | 고규영 | - |
dc.contributor.author | Choi. Dong-Kyu | - |
dc.contributor.author | 최동규 | - |
dc.date.accessioned | 2013-09-11T01:05:21Z | - |
dc.date.available | 2013-09-11T01:05:21Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=513587&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/179810 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2013.2, [ vi, 90 p. ] | - |
dc.description.abstract | Successful differentiation and expansion of endothelial cells (ECs) from embryonic stem cell (ESC)-derived Flk1+ mesodermal precursor cells (MPCs) requires supplementation of vascular endothelial growth factor-A (VEGF-A). While analyzing VEGF-A/VEGFR2 downstream signaling pathway that underlies the VEGF-A-induced differentiation and ex-pansion of ECs, we fortuitously found that Rho-associated protein kinase (ROCK) inhibitor Y27632 profoundly promoted the differentiation and expansion of ECs from Flk1+ MPCs, while reducing the differentiation and expansion of mural cells. The ROCK suppression-induced expansion of ECs appears to have resulted from promotion of proliferation of ECs via activation of PI3-kinase-Akt signaling. The ECs obtained by the combination of ROCK suppression and VEGF-A supplementation faithfully expressed most pan-EC surface mak-ers, and phenotypic analyses revealed that they were differentiated toward arterial EC. Fur-ther incubation of the ICAM2+ ECs with Y27632 and VEGF-A for 2 days promoted expansion of ECs by 6.5-fold compared to those incubated with only VEGF-A. Importantly, the ROCK suppression-induced ECs displayed neovasculogenic abilities in vitro and in vivo. Thus, supplementation of ROCK inhibitor Y27632 along with VEGF-A in 2D Matrigel culture system provides a simple, efficient, and versatile method for obtaining ample amount of ESC-derived ECs at high purity suitable for use in therapeutic neovascularization. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | embryonic stem cell | - |
dc.subject | endothelial cell | - |
dc.subject | VEGF-A | - |
dc.subject | mural cell | - |
dc.subject | 배아 줄기 세포 | - |
dc.subject | 혈관 내피 세포 | - |
dc.subject | 혈관 벽 세포 | - |
dc.subject | ROCK | - |
dc.title | Role of ROCK signaling on endothelial differentiation of mouse embryonic stem cell | - |
dc.title.alternative | 배아줄기세포의 혈관 내피 세포 분화 중 ROCK 신호 전달 체계의 역할 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 513587/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020095166 | - |
dc.contributor.localauthor | Koh, Gou-Young | - |
dc.contributor.localauthor | 고규영 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.