DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, EC | ko |
dc.contributor.author | Yun, JW | ko |
dc.contributor.author | Jung, KH | ko |
dc.contributor.author | Chung, SI | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2010-04-14T07:09:16Z | - |
dc.date.available | 2010-04-14T07:09:16Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-11 | - |
dc.identifier.citation | BIOPROCESS ENGINEERING, v.19, no.5, pp.363 - 372 | - |
dc.identifier.issn | 0178-515X | - |
dc.identifier.uri | http://hdl.handle.net/10203/17720 | - |
dc.description.abstract | A perfusion-control strategy based on cellular consumption rates of oxygen and glucose was established for the production of single-chain urokinase-type plasminogen activator (scu-PA). Employing this strategy, the influences of microcarrier types and the culture media on culture performances were evaluated. In the control perfusion culture, which used a solid microcarrier and a 1% fetal bovine serum (FBS) medium, viable cell density reached 3.1 x 10(7) cells ml(-1). However, formation of large, heterogeneous aggregates (500-1,000 mu m) resulted in a gradual decrease in viable cell density to less than 1.0 x 10(7) cells ml(-1). Accordingly, declines in the production of urokinase-type plasminogen activator (u-PA) and in the scu-PA portion of u-PA were observed. In the serum-free media, cell growth and u-PA production were suppressed 2-3 times, but were significantly enhanced when a porous microcarrier, Cultispheer G, was used. The cell-growth profile showed a continuous increase in cell density, reaching 5.1 x 10(7) cells ml(-1), and the production of u-PA remained stable throughout the culture (1586 +/- 247 IU ml(-1)). The values of all the parameters associated with cell growth and u-PA production were fairly comparable to or even higher than those in the control culture. Moreover, a 13% higher scu-PA portion of u-PA was observed in the serum-free culture, regardless of the microcarrier type, compared with scu-PA portion of u-PA in the control culture. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER VERLAG | - |
dc.subject | SERUM-FREE MEDIUM | - |
dc.subject | CELL-CULTURE | - |
dc.subject | DISSOLVED-OXYGEN | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | CHO CELLS | - |
dc.subject | PROUROKINASE | - |
dc.subject | AFFINITY | - |
dc.subject | GLUCOSE | - |
dc.subject | GROWTH | - |
dc.subject | FIBRIN | - |
dc.title | Performance study of perfusion cultures for the production of single-chain urokinase-type plasminogen activator (scu-PA) in a 2.5 l spin-filter bioreactor | - |
dc.type | Article | - |
dc.identifier.wosid | 000077303400007 | - |
dc.identifier.scopusid | 2-s2.0-85047678943 | - |
dc.type.rims | ART | - |
dc.citation.volume | 19 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 363 | - |
dc.citation.endingpage | 372 | - |
dc.citation.publicationname | BIOPROCESS ENGINEERING | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Jo, EC | - |
dc.contributor.nonIdAuthor | Yun, JW | - |
dc.contributor.nonIdAuthor | Jung, KH | - |
dc.contributor.nonIdAuthor | Chung, SI | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SERUM-FREE MEDIUM | - |
dc.subject.keywordPlus | CELL-CULTURE | - |
dc.subject.keywordPlus | DISSOLVED-OXYGEN | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CHO CELLS | - |
dc.subject.keywordPlus | PROUROKINASE | - |
dc.subject.keywordPlus | AFFINITY | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | FIBRIN | - |
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