DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Joon-Goo | ko |
dc.contributor.author | Lee, Yong Jae | ko |
dc.contributor.author | Velmurugan, Natarajan | ko |
dc.contributor.author | Ko, Young-Joon | ko |
dc.contributor.author | Lee, Hyang-Sim | ko |
dc.contributor.author | Jeong, Kijun | ko |
dc.date.accessioned | 2013-08-14T01:09:33Z | - |
dc.date.available | 2013-08-14T01:09:33Z | - |
dc.date.created | 2013-08-09 | - |
dc.date.created | 2013-08-09 | - |
dc.date.issued | 2013-07 | - |
dc.identifier.citation | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.40, no.7, pp.705 - 713 | - |
dc.identifier.issn | 1367-5435 | - |
dc.identifier.uri | http://hdl.handle.net/10203/175031 | - |
dc.description.abstract | For effective control of foot-and-mouth disease (FMD), the development of rapid diagnostic systems and vaccines are required against its etiological agent, FMD virus (FMDV). To accomplish this, efficient large-scale expression of the FMDV VP1 protein, with high solubility, needs to be optimized. We attempted to produce high levels of a serotype O FMDV VP1 epitope in Escherichia coli. We identified the subtype-independent serotype O FMDV VP1 epitope sequence and used it to construct a glutathione S-transferase (GST) fusion protein. For efficient production of the FMDV VP1 epitope fused to GST (VP1e-GST), four E. coli strains and three temperatures were examined. The conditions yielding the greatest level of VP1e-GST with highest solubility were achieved with E. coli BL21(DE3) at 25 A degrees C. For high-level production, fed-batch cultures were conducted in 5-l bioreactors. When cells were induced at a high density and complex feeding solutions were supplied, approximately 11 g of VP1e-GST was obtained from a 2.9-l culture. Following purification, the VP1 epitope was used to immunize rabbits, and we confirmed that it induced an immune response. | - |
dc.language | English | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | G-H LOOP | - |
dc.subject | HIGH-LEVEL PRODUCTION | - |
dc.subject | HUMAN LEPTIN | - |
dc.subject | EXPRESSION | - |
dc.subject | BATCH | - |
dc.subject | CULTIVATION | - |
dc.subject | CULTURE | - |
dc.subject | ANTIGEN | - |
dc.subject | SWINE | - |
dc.title | High-yield production of the VP1 structural protein epitope from serotype O foot-and-mouth disease virus in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000320396000006 | - |
dc.identifier.scopusid | 2-s2.0-84879208105 | - |
dc.type.rims | ART | - |
dc.citation.volume | 40 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 705 | - |
dc.citation.endingpage | 713 | - |
dc.citation.publicationname | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s10295-013-1273-7 | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.nonIdAuthor | Jung, Joon-Goo | - |
dc.contributor.nonIdAuthor | Lee, Yong Jae | - |
dc.contributor.nonIdAuthor | Velmurugan, Natarajan | - |
dc.contributor.nonIdAuthor | Ko, Young-Joon | - |
dc.contributor.nonIdAuthor | Lee, Hyang-Sim | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | Fed-batch cultivation | - |
dc.subject.keywordAuthor | FMDV | - |
dc.subject.keywordAuthor | VP1 epitope | - |
dc.subject.keywordPlus | G-H LOOP | - |
dc.subject.keywordPlus | HIGH-LEVEL PRODUCTION | - |
dc.subject.keywordPlus | HUMAN LEPTIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BATCH | - |
dc.subject.keywordPlus | CULTIVATION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | SWINE | - |
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