DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cui, Chang-Hao | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.date.accessioned | 2013-08-08T05:45:51Z | - |
dc.date.available | 2013-08-08T05:45:51Z | - |
dc.date.created | 2013-02-28 | - |
dc.date.created | 2013-02-28 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.97, no.2, pp.649 - 659 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/174596 | - |
dc.description.abstract | This study focused on the cloning, expression, and characterization of ginsenoside-transforming recombinant beta-glucosidase from Actinosynnema mirum KACC 20028(T) in order to biotransform ginsenosides efficiently. The gene, termed as bglAm, encoding a beta-glucosidase (BglAm) belonging to the glycoside hydrolase family 3 was cloned. bglAm consisted of 1,830 bp (609 amino acid residues) with a predicted molecular mass of 65,277 Da. This enzyme was overexpressed in Escherichia coli BL21(DE3) using a GST-fused pGEX 4T-1 vector system. The recombinant BglAm was purified with a GST center dot bind agarose resin and characterized. The optimum conditions of the recombinant BglAm were pH 7.0 and 37 A degrees C. BglAm could hydrolyze the outer and inner glucose moieties at the C3 and C20 of the protopanaxadiol-type ginsenosides (i.e., Rb-1 and Rd, gypenoside XVII) to produce protopanaxadiol via gypenoside LXXV, F-2, and Rh-2(S) with various pathways. BglAm can effectively transform the ginsenoside Rb-1 to gypenoside XVII and Rd to F-2; the K (m) values of Rb-1 and Rd were 0.69 A +/- 0.06 and 0.45 A +/- 0.02 mM, respectively, and the V (max) values were 16.13 A +/- 0.29 and 51.56 A +/- 1.35 mu mol min(-1) mg(-1) of protein, respectively. Furthermore, BglAm could convert the protopanaxatriol-type ginsenoside Re and Rg(1) into Rg(2)(S) and Rh-1(S) hydrolyzing the attached glucose moiety at the C6 and C20 positions, respectively. These various ginsenoside-hydrolyzing pathways of BglAm may assist in producing the minor ginsenosides from abundant major ginsenosides. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | PROTOPANAXATRIOL-TYPE GINSENOSIDES | - |
dc.subject | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject | HYDROLYZING 6-O-MULTI-GLYCOSIDES | - |
dc.subject | 20(S)-GINSENOSIDE RG3 | - |
dc.subject | COLORECTAL-CANCER | - |
dc.subject | DIFFERENT PARTS | - |
dc.subject | PANAX-GINSENG | - |
dc.subject | COMPOUND K | - |
dc.subject | BIOTRANSFORMATION | - |
dc.subject | GLYCOSIDASE | - |
dc.title | Characterization of the ginsenoside-transforming recombinant beta-glucosidase from Actinosynnema mirum and bioconversion of major ginsenosides into minor ginsenosides | - |
dc.type | Article | - |
dc.identifier.wosid | 000313651700016 | - |
dc.identifier.scopusid | 2-s2.0-84873990694 | - |
dc.type.rims | ART | - |
dc.citation.volume | 97 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 649 | - |
dc.citation.endingpage | 659 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s00253-012-4324-5 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Cui, Chang-Hao | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Ginsenoside | - |
dc.subject.keywordAuthor | Biotransformation | - |
dc.subject.keywordAuthor | Glycoside hydrolase | - |
dc.subject.keywordAuthor | Actinosynnema mirum | - |
dc.subject.keywordPlus | PROTOPANAXATRIOL-TYPE GINSENOSIDES | - |
dc.subject.keywordPlus | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject.keywordPlus | HYDROLYZING 6-O-MULTI-GLYCOSIDES | - |
dc.subject.keywordPlus | 20(S)-GINSENOSIDE RG3 | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | DIFFERENT PARTS | - |
dc.subject.keywordPlus | PANAX-GINSENG | - |
dc.subject.keywordPlus | COMPOUND K | - |
dc.subject.keywordPlus | BIOTRANSFORMATION | - |
dc.subject.keywordPlus | GLYCOSIDASE | - |
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