DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yeon-Gu | ko |
dc.contributor.author | Park, Byoungwoo | ko |
dc.contributor.author | Lee, Seungwoon | ko |
dc.contributor.author | Ahn, Jung Oh | ko |
dc.contributor.author | Jung, Joon-Ki | ko |
dc.contributor.author | Lee, Hong Weon | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.contributor.author | Lee, Eun Gyo | ko |
dc.date.accessioned | 2013-06-07T08:17:40Z | - |
dc.date.available | 2013-06-07T08:17:40Z | - |
dc.date.created | 2012-08-13 | - |
dc.date.created | 2012-08-13 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.citation | PROCESS BIOCHEMISTRY, v.47, no.12, pp.2518 - 2522 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/10203/173888 | - |
dc.description.abstract | Overexpression of Bcl-2, a typical anti-apoptotic protein, is one of the most effective means to maintain mitochondria integrity in recombinant CHO (rCHO) cell culture treated with sodium butyrate (NaBu). NaBu is known as a typical specific productivity-enhancing factor and also a well-known apoptosis inducer. Bcl-2 is distributed to and functions in multiple intracellular organelles such as the nucleus, mitochondria, and endoplasmic reticulum (ER). To evaluate the effect of organelle-specific overexpression of Bcl-2 on NaBu-induced apoptosis in rCHO cells, Bcl-2 expression was restricted to the mitochondria or to the ER either by employing a mitochondrial insertion sequence of ActA or by insertion of an ER-specific sequence of cytochrome b5 to their respective sequences. The rCHO cell lines overexpressing wild-type Bcl-2 (WT-Bcl-2), mitochondrial Bcl-2 (MT-Bcl-2), and ER-targeted Bcl-2 (ER-Bcl-2) were established. Overexpression of WT-Bcl-2, MT-Bcl-2, and ER-Bcl-2 could increase cell viability and decrease LDH release under NaBu-treated conditions. Additionally, overexpression of WT-Bcl-2, MT-Bcl-2, and ER-Bcl-2 could suppress NaBu-induced apoptosis, as demonstrated by a DNA fragmentation assay. A mitochondrial membrane potential assay revealed that ER-Bcl-2 overexpression can maintain the mitochondrial membrane integrity without being affected by MT-Bcl-2 overexpression, indicating that the role of ER should be considered in alleviating NaBu-induced apoptosis by a genetic modulation strategy. Taken together, it was found that restricted Bcl-2 overexpression at the ER can inhibit the NaBu-induced apoptosis by maintaining mitochondria integrity in rCHO cells. (C) 2012 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | ENDOPLASMIC-RETICULUM | - |
dc.subject | DEATH | - |
dc.subject | PROTEINS | - |
dc.subject | AUTOPHAGY | - |
dc.title | Effect of mitochondrial and ER-targeted Bcl-2 overexpression on apoptosis in recombinant Chinese hamster ovary cells treated with sodium butyrate | - |
dc.type | Article | - |
dc.identifier.wosid | 000313851700108 | - |
dc.identifier.scopusid | 2-s2.0-84870850341 | - |
dc.type.rims | ART | - |
dc.citation.volume | 47 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 2518 | - |
dc.citation.endingpage | 2522 | - |
dc.citation.publicationname | PROCESS BIOCHEMISTRY | - |
dc.identifier.doi | 10.1016/j.procbio.2012.06.021 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Kim, Yeon-Gu | - |
dc.contributor.nonIdAuthor | Park, Byoungwoo | - |
dc.contributor.nonIdAuthor | Lee, Seungwoon | - |
dc.contributor.nonIdAuthor | Ahn, Jung Oh | - |
dc.contributor.nonIdAuthor | Jung, Joon-Ki | - |
dc.contributor.nonIdAuthor | Lee, Hong Weon | - |
dc.contributor.nonIdAuthor | Lee, Eun Gyo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Sodium butyrate | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Mitochondrial Bcl-2 | - |
dc.subject.keywordAuthor | ER-targeted Bcl-2 | - |
dc.subject.keywordAuthor | rCHO cells | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
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