Grephene-based electrode utilized with Fusion Protein for Detection of Organophosphate Pesticides

Abstract

Herein, we demonstrated an immobilization strategy for high biological activities and high performed biosensors without conformational changes by using bioconjugates consisted of fusion proteins and reduced graphene oxide-gold nanoparticle hybrid materials (RGO-Au). The GBP-OPH/RGO-Au nanobio-conjugates were expected that hybrid systems enable to improve enzyme stability, and biocatalytic activity, and electron transfer over the enzymatic reaction, which attributed to favorable morphology of immobilized GBP-OPH. The particle size and distribution of Au on RGO were characterized by TEM and AFM images. The electrochemical properties of GBP-OHP/Au-RGO hybrids were analyzed by cyclic voltametry and impedance analysis. The amperometric measurements were performed for detection of OP compounds by flow injection method.