Development of succinic acid-tolerant Mannheimia succiniciproducens based on transcriptome analysis

Abstract

A genetically engineered M. succiniciproducens strain for succinic production was adaptively evolved for improved succinic acid tolerance. Immediate and delayed responses of the parent strain and the mutant strain to succinic acid shock were analyzed in transcriptome level by DNA microarray. Gene expression levels of the mutant strain were compared with a parent strain and the gene expression patterns were classified refer to function of genes. Overexpression of gene encoding the carboxylic acid transporter selected based on the transcriptome analysis increased growth rate of the parent strain in the medium with succinic acid of high concentration.