Microcontact Printing of Biotin for Selective Immobilization of Streptavidin-Fused Proteins and Cells

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dc.contributor.author최솔ko
dc.contributor.author박태정ko
dc.contributor.authorLee, Kko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2013-03-28T04:54:01Z-
dc.date.available2013-03-28T04:54:01Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2007-04-
dc.identifier.citationAnnual Spring Meeting of KIChE, pp.422-
dc.identifier.urihttp://hdl.handle.net/10203/162948-
dc.description.abstractA simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (μCP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. As a proof-of-principle, we constructed Bacillus spores that displayed EGFP on the spore surface. The results suggest that this strategy is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications.-
dc.languageKorean-
dc.publisherAnnual Spring Meeting of KIChE-
dc.titleMicrocontact Printing of Biotin for Selective Immobilization of Streptavidin-Fused Proteins and Cells-
dc.typeConference-
dc.type.rimsCONF-
dc.citation.beginningpage422-
dc.citation.publicationnameAnnual Spring Meeting of KIChE-
dc.identifier.conferencecountryKO-
dc.identifier.conferencelocation울산 롯데호텔-
dc.contributor.localauthorLee, SangYup-
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CBE-Conference Papers(학술회의논문)
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