DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최솔 | ko |
dc.contributor.author | 박태정 | ko |
dc.contributor.author | Lee, K | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2013-03-28T04:54:01Z | - |
dc.date.available | 2013-03-28T04:54:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-04 | - |
dc.identifier.citation | Annual Spring Meeting of KIChE, pp.422 | - |
dc.identifier.uri | http://hdl.handle.net/10203/162948 | - |
dc.description.abstract | A simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (μCP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. As a proof-of-principle, we constructed Bacillus spores that displayed EGFP on the spore surface. The results suggest that this strategy is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications. | - |
dc.language | Korean | - |
dc.publisher | Annual Spring Meeting of KIChE | - |
dc.title | Microcontact Printing of Biotin for Selective Immobilization of Streptavidin-Fused Proteins and Cells | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.citation.beginningpage | 422 | - |
dc.citation.publicationname | Annual Spring Meeting of KIChE | - |
dc.identifier.conferencecountry | KO | - |
dc.identifier.conferencelocation | 울산 롯데호텔 | - |
dc.contributor.localauthor | Lee, SangYup | - |
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