A major challenge in cell replacement therapy for neurological diseases is the sorting of differentiated neurons because conventional cell sorting techniques require cells in suspension. Recently, spherical glass beads have been demonstrated as a movable growth substrate, allowing transport of differentiated neurons without dissociation. Phototransistor-based optoelectronic tweezers provides a gentle mean to optically manipulate cells. By combining these two approaches, we demonstrated fluorescent-activated automatic sorting of differentiated neurons. We envision employing this cell sorting system for cell replacement therapy to treat neurodegenerative diseases and other nerve injuries.