DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, BH | ko |
dc.contributor.author | Lee, JH | ko |
dc.contributor.author | Kim, M | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.date.accessioned | 2010-01-08T06:28:22Z | - |
dc.date.available | 2010-01-08T06:28:22Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-02 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.268, no.1, pp.136 - 140 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/16230 | - |
dc.description.abstract | Escherichia coli RNase P, an RNA-processing enzyme that cleaves precursor tRNAs to generate the mature 5'-end, is composed of a catalytic component (M1 RNA) and a protein cofactor (C5 protein). In this study, effects of C5 protein on the RNase P catalysis with a precursor E. coli tRNA(Phe) having a single mismatch in the acceptor stem were examined. This mutant precursor unexpectedly generated upstream cleavage products at the -8 position as well as normal cleavage products at the +1 position. The cleavage at the -8 position was essentially effective only in the presence of C5 protein. Possible secondary structures for cleavage at the -8 position deviate significantly from the structures of the known RNase P substrates, implying that C5 protein can allow the enzyme to broaden the substrate specificity more than previously appreciated, (C) 2000 Academic Press. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ACADEMIC PRESS INC | - |
dc.subject | PHOTOAFFINITY CROSS-LINKING | - |
dc.subject | 4.5 S-RNA | - |
dc.subject | RIBONUCLEASE-P | - |
dc.subject | SUBSTRATE | - |
dc.subject | RIBOZYME | - |
dc.subject | SUBUNIT | - |
dc.subject | MUTANTS | - |
dc.subject | ENZYME | - |
dc.subject | SEQUENCE | - |
dc.title | Effects of C5 protein on Escherichia coli RNase P catalysis with a precursor tRNA(Phe) bearing a single mismatch in the acceptor stem | - |
dc.type | Article | - |
dc.identifier.wosid | 000085173500027 | - |
dc.identifier.scopusid | 2-s2.0-0034607090 | - |
dc.type.rims | ART | - |
dc.citation.volume | 268 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 136 | - |
dc.citation.endingpage | 140 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Park, BH | - |
dc.contributor.nonIdAuthor | Lee, JH | - |
dc.contributor.nonIdAuthor | Kim, M | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | RNase P | - |
dc.subject.keywordAuthor | C5 protein | - |
dc.subject.keywordAuthor | M1 RNA | - |
dc.subject.keywordAuthor | substrate mutation | - |
dc.subject.keywordAuthor | precursor tRNA(Phe) | - |
dc.subject.keywordPlus | PHOTOAFFINITY CROSS-LINKING | - |
dc.subject.keywordPlus | 4.5 S-RNA | - |
dc.subject.keywordPlus | RIBONUCLEASE-P | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | RIBOZYME | - |
dc.subject.keywordPlus | SUBUNIT | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | SEQUENCE | - |
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